New Trypanosoma cruzi repeated element that shows site specificity for insertion.
New Trypanosoma cruzi repeated element that shows site specificity for insertion.
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新的克氏锥虫重复元件显示插入位点特异性。
DOI：
10.1128/ec.00036-07
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
daSilveira,JoséFranco
中科院分区：
文献类型：
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作者：
Souza,RenataT;Santos,MárciaRM;Lima,FábioM;El-Sayed,NajibM;Myler,PeterJ;Ruiz,JeronimoC;daSilveira,JoséFranco
本文描述了在克氏锥虫中发现的一个新的位点特异性重复元件家族，我们将其命名为TcTREZO。TcTREZO似乎是一个复合重复元件，因为可以根据与其他T的序列相似性在其内部定义三个亚区。CRUZISE序列。对TcTREZO在基因组中的分布分析清楚地表明，它具有插入的位点特异性。大多数TcTREZO元件的两侧都有保守序列。在该元件的5‘端有一个高度保守的68bp的序列，在3’端有一个∼500bp的序列结构域，没有明确的边界线。Northern杂交和逆转录酶PCR分析表明，TcTREZO转录本表达为寡聚(A)终止的转录本，其长度与该元件的单位大小(1.6kb)相对应。在稳态∼中也检测到来自一小部分TcTREZO的0.2kb的转录本。TcTREZO转录产物是非剪接的，也不是翻译的。TcTREZO序列的拷贝数估计为每个单倍体基因组的∼为173个拷贝。TcTREZO似乎是通过将序列插入到前体元件中组装而成的。一旦相互关联，这些亚基就被扩增为一种新的转座元件。TcTREZO显示了插入的位置特异性，表明序列特异性内切酶可能负责其在唯一位置的插入。
A new family of site-specific repeated elements identified inTrypanosoma cruzi, which we named TcTREZO, is described here. TcTREZO appears to be a composite repeated element, since three subregions may be defined within it on the basis of sequence similarities with otherT. cruzisequences. Analysis of the distribution of TcTREZO in the genome clearly indicates that it displays site specificity for insertion. Most TcTREZO elements are flanked by conserved sequences. There is a highly conserved 68-bp sequence at the 5′ end of the element and a sequence domain of ∼500 bp without a well-defined borderline at the 3′ end. Northern blot hybridization and reverse transcriptase PCR analyses showed that TcTREZO transcripts are expressed as oligo(A)-terminated transcripts whose length corresponds to the unit size of the element (1.6 kb). Transcripts of ∼0.2 kb derived from a small part of TcTREZO are also detected in steady-state RNA. TcTREZO transcripts are unspliced and not translated. The copy number of TcTREZO sequences was estimated to be ∼173 copies per haploid genome. TcTREZO appears to have been assembled by insertions of sequences into a progenitor element. Once associated with each other, these subunits were amplified as a new transposable element. TcTREZO shows site specificity for insertion, suggesting that a sequence-specific endonuclease could be responsible for its insertion at a unique site.