Comparison of the p26 gene region of two baculoviruses.
Comparison of the p26 gene region of two baculoviruses.
复制标题
两种杆状病毒p26基因区域的比较。
DOI：
10.1016/0042-6822(87)90155-3
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Beaudreau,GS
中科院分区：
文献类型：
--
作者：
Bicknell,JN;Leisy,DJ;Rohrmann,GF;Beaudreau,GS
A 1.1-kb region of DNA containing the p26 gene of the multicapsid nuclear polyhedrosis virus ofOrgyia pseudotsugata(OpMNPV) was sequenced, transcriptionally mapped, and compared to the same region in the MNPV ofAutographa californica(AcMNPV). The mRNA start site of the p26 gene occurs about 22 nucleotides downstream from an A/T-rich putative promoter sequence that is highly conserved between AcMNPV and OpMNPV. The p26 mRNA is transcribed through the p26 gene and coterminates with the p10 gene resulting in a mRNA containing copies of both genes. The reading frames of the OpMNPV and AcMNPV p26 genes showed 47% amino acid sequence homology which is clustered in six regions with over 65% amino acid homology. There was a distinct bias toward incorporation of G/C-rich codons in the OpMNPV p26 gene. No DNA homology was observed between the region upstream of the p26 gene in AcMNPV and OpMNPV. In AcMNPV, this region contains the homologous repeated (hr) sequencehr5. Hybridization of a plasmid containing an AcMNPV-repeated sequence (hr5) to Southern blots of the OpMNPV genome indicated that this repeated sequence is lacking in OpMNPV.
