Bioremediation of heavy metals by using bacterial mixtures
Bioremediation of heavy metals by using bacterial mixtures
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DOI：
10.1016/j.ecoleng.2016.01.023
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发表时间：
2016-04-01
影响因子：
3.8
通讯作者：
So, Jae-Seong
中科院分区：
文献类型：
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作者：
Kang, Chang-Ho;Kwon, Yoon-Jung;So, Jae-Seong
由于采矿、冶金过程和其他化学工业造成的重金属和有毒金属对环境的污染是一个影响人类健康和环境的世界性问题。本研究的目的是探讨细菌混合物对污染土壤中铅、镉和铜混合物的生物修复的协同效应。与单一培养法相比，细菌混合物具有更高的生长速度、脲酶活性和对重金属的抗性。从韩国某废弃矿区获得的混合菌群中分离鉴定出4株细菌，其中砂绿芽孢杆菌B-21、梭孢芽孢杆菌B-22、阴沟肠杆菌KJ-46和阴沟肠杆菌KJ-47表现出有效的微生物诱导方解石沉淀（MICP）。在实验过程中监测了以下参数：光密度、pH、脲酶活性、方解石产量、重金属耐受性和抗渗性测试。通过对细菌混合物的改性，观察到对多种重金属的协同修复效果，48 h后，Pb的修复率为98.3%，Cd的修复率为85.4%，Cu的修复率为5.6%。与单菌种培养相比，混合菌对重金属的抗性和修复效率更高。因此，我们的研究结果表明，细菌混合物的使用在污染环境中重金属的生物修复中是有用的。(C) 2016 Elsevier B.V.版权所有
Environmental pollution by heavy and toxic metals because of mining, metallurgic processes, and other chemical industries is a worldwide problem affecting both human health and the environment. The aim of this study was to investigate the synergistic effect of bacterial mixtures on the bioremediation of a mixture of Pb, Cd, and Cu from contaminated soils. Compared to the single culture method, the bacterial mixtures showed higher growth rate, urease activity, and resistance to heavy metals. Four bacterial strains were isolated and identified from bacterial mixtures Viridibacillus arenosi B-21, Sporosarcina soli B-22, Enterobacter cloacae KJ-46, and E. cloacae KJ-47, which obtained from an abandoned mine site in Korea and showed effective microbially induced calcite precipitation (MICP). The following parameters were monitored during the course of the experiment: optical density, pH, urease activity, calcite production, tolreance to heavy metals, and impermeability test. Synergistic effects on the remediation of various heavy metals via modification of the bacterial mixtures were observed and, after 48 h, remediation of 98.3% for Pb, 85.4% for Cd, and 5.6% for Cu were recorded. Compared with single strain cultures, the bacterial mixtures demonstrated greater resistance and efficiency for the remediation of heavy metals. Thus, our results show that the use of bacterial mixtures is useful in the bioremediation of heavy metals from the contaminated environment. (C) 2016 Elsevier B.V. All rights reserved.