Regulation of an epithelial chloride channel by direct phosphorylation and dephosphorylation.
Regulation of an epithelial chloride channel by direct phosphorylation and dephosphorylation.
复制标题
通过直接磷酸化和去磷酸化调节上皮氯离子通道。
DOI：
10.1152/ajpcell.1992.263.1.c172
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Brautigan,DL
中科院分区：
文献类型：
--
作者：
Finn,AL;Gaido,ML;Dillard,M;Brautigan,DL
天然氯离子通道在Necturus胆囊上皮细胞开放的茶碱诱导的细胞环AMP的上升，并通过去除茶碱或通过添加特异性抗体关闭;然而，它不会关闭，如果冈田酸，蛋白磷酸酶1和2A的抑制剂，被添加。重组成脂质双层的纯化通道在加入蛋白磷酸酶2A后关闭，并通过加入Mg-ATP和环AMP依赖性蛋白激酶的催化亚基重新打开。这些结果表明，通道蛋白是在磷酸化状态下纯化的，其功能特性至少部分由直接磷酸化和去磷酸化控制。
A native chloride channel in Necturus gallbladder epithelial cells is opened by a theophylline-induced rise in cellular cyclic AMP and is closed by removal of theophylline or by addition of specific antibody; however, it does not close if okadaic acid, an inhibitor of protein phosphatases 1 and 2A, is added. The purified channel reconstituted into lipid bilayers closes upon the addition of protein phosphatase 2A and is reopened by the addition of Mg-ATP and the catalytic subunit of cyclic AMP-dependent protein kinase. These results indicate that the channel protein is purified in a phosphorylated state and that its functional characteristics are at least partly controlled by direct phosphorylation and dephosphorylation.