Increased expression of vasoactive intestinal polypeptide in cultured myenteric neurons from adult rat small intestine
Increased expression of vasoactive intestinal polypeptide in cultured myenteric neurons from adult rat small intestine
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DOI：
10.1016/s1566-0702(03)00077-8
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发表时间：
2003-08-29
影响因子：
2.7
通讯作者：
Ekblad, E
中科院分区：
文献类型：
--
作者：
Lin, Z;Sandgren, K;Ekblad, E
成年神经元具有适应环境变化的能力。已知由于轴突切断或通过培养分离而导致靶源性神经营养因子的损失会诱导几种类型的外周神经元中神经肽表达的变化。本研究的目的是探讨血管活性肠肽（VIP）和一氧化氮合酶（NOS）在培养的肌间神经节和分离的神经元表达的变化。分离大鼠小肠的肌间神经节和神经元并培养长达21天。免疫细胞化学用于确定神经元的总数和含有VIP或NOS或两者的亚群的比例，在整个坐骑的制剂（用于确定体内条件的对照），肌间神经节培养物和解离的肌间神经元培养物。原位杂交法检测NOS和VIP mRNA表达的变化。VIP表达神经元的相对数量在培养过程中显着增加。所有表达VIP的神经元的百分比在整个组织中为3.6 +/- 0.3%，在培养的肌间神经节中为22-24%，在培养的分离神经元中高达35%。NOS表达的神经元在整个坐骑以及在培养的神经节或分离的神经元中占所有神经元的约30-40%。NOS/VIP神经元的显着增加，检测培养的神经元，无论它们是否被安排在神经节或解离相比，整个安装准备。这表明含NOS的神经元是增加其VIP表达的神经元。VIP在培养的成人肌间神经元中的诱导表达表明VIP对神经元的适应、维持和存活是重要的。(C)2003 Elsevier Science B. V.保留所有权利。
Adult neurons possess the ability to adapt to a changing environment. Loss of target-derived neurotrophic factors due to axotomy or isolation by culturing is known to induce changes in neuropeptide expression in several types of peripheral neurons. The aim of the present study was to investigate changes in the expression of vasoactive intestinal polypeptide (VIP) and nitric oxide synthase (NOS) in cultured myenteric ganglia and dissociated neurons. Myenteric ganglia and neurons from rat small intestine were dissociated and cultured for up to 21 days. Immunocytochemistry was used to determine the total number of neurons and the proportions of subpopulations containing VIP or NOS or both in preparations of whole mounts (controls used to determine the conditions in vivo), myenteric ganglion culture and dissociated myenteric neuronal culture. In situ hybridization was used to determine changes in the expressions of NOS and VIP mRNA. The relative number of VIP-expressing neurons increased significantly during culturing. The percentage of all neurons expressing VIP was 3.6 +/- 0.3% in whole mounts, 22-24% in cultured myenteric ganglia, and up to 35% in cultured dissociated neurons. NOS-expressing neurons constituted approximately 30-40% of all neurons in whole mounts as well as in cultured ganglia or dissociated neurons. A dramatic increase in NOS/VIP-containing neurons were detected in cultured neurons irrespective of whether they were arranged in ganglia or dissociated as compared to whole mount preparations. This suggests that the NOS-containing neurons are the ones that increase their VIP expression. The induced expression of VIP in cultured adult myenteric neurons indicates that VIP is important for neuronal adaptation, maintenance and survival. (C) 2003 Elsevier Science B.V. All rights reserved.