Detection of human papillomavirus-16 DNA in archived clinical samples of breast and lung cancer patients from North Pakistan
Detection of human papillomavirus-16 DNA in archived clinical samples of breast and lung cancer patients from North Pakistan
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DOI：
10.1007/s00432-016-2251-z
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发表时间：
2016-12-01
影响因子：
3.6
通讯作者：
Murad, Sheeba
中科院分区：
文献类型：
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作者：
Ilahi, Naureen Ehsan;Anwar, Sobia;Murad, Sheeba
在过去的几十年里，人乳头瘤病毒（HPV）已被记录为各种生殖器癌症，最值得注意的是宫颈癌发展的关键参与者。它也与一些非生殖器癌症有关。已知口咽癌的一个子集是由HPV引起的。乳腺癌和肺癌的病因学也被认为与乳腺癌和肺癌有关。然而，关于HPV DNA检测的报告在世界不同地区差异很大。本研究收集了我国北方地区46例乳腺癌和9例肺浸润性导管癌的石蜡包埋组织标本共55例，采用回顾性方法检测HPV的感染情况。采用PCR方法对HPV 16和18进行基因分型，乳腺癌和肺癌组织中均检出HPV 16 DNA，检出率分别为17%和11%。ER/PR（雌激素/孕激素受体）和HER 2/Neu（人表皮生长因子受体-2）阳性与乳腺肿瘤中HPV的发生之间存在有趣的关联。目前的研究显示，在乳腺癌和肺癌的存档临床活检切片中，HPV 16 DNA的存在分别为17，11%。HPV 16的存在与ER/PR和HER 2阳性乳腺癌呈正相关。这些初步发现值得进一步研究，以确定HPV的患病率和病因在当地癌症中的作用，特别是在更大规模的ER/PR/HER 2阳性乳腺癌中。
Over the past few decades, human papillomavirus (HPV) has been recorded as a key player in the development of various genital cancers, most notably cervical cancer. It has also been associated with some non-genital cancers. A subset of oropharyngeal cancers are known to be caused by HPV. Its aetiological involvement has been suggested for breast and lung cancer as well. However, reports regarding the HPV DNA detection vary widely from different parts of the world. Due to scarcity of local data in this regard, the current study aimed at retrospective detection of HPV presence in the archival samples of breast and lung cancer patients from north part of the country.A total of 55 formalin-fixed paraffin-embedded tissue sections of invasive ductal carcinoma of breast (n = 46) and lung (n = 9) were collected for this study. Genotyping for HPV16 and 18 was carried out through PCR.HPV16 DNA was found in both breast and lung carcinoma samples with the prevalence rate of 17 and 11 %, respectively. An interesting association was found between ER/PR (Oestrogen/Progesterone receptor) and HER2/Neu (Human epidermal growth factor receptor-2) positivity with HPV occurrence in breast tumours.Current study shows the presence of HPV16 DNA in archived clinical biopsy sections from breast and lung cancers (17, 11 %), respectively. A positive correlation of HPV16 presence was found with ER/PR and HER2-positive breast cancers. These initial findings warrant further investigation in order to determine HPV prevalence and aetiological role in local cancers, especially in ER/PR/HER2-positive breast cancers on a larger scale.