Low-dose phase retrieval of biological specimens using cryo-electron ptychography
Low-dose phase retrieval of biological specimens using cryo-electron ptychography
复制标题
使用冷冻电子叠层成像技术对生物样本进行低剂量相检索
DOI：
10.1038/s41467-020-16391-6
复制
发表时间：
2020-06-02
影响因子：
16.6
通讯作者：
Wang, Peng
中科院分区：
文献类型：
--
作者：
Zhou, Liqi;Song, Jingdong;Wang, Peng
低温电子显微镜是研究生物系统高分辨率结构的重要工具。这种方法依赖于在高离焦时使用相衬成像，以牺牲较高的空间频率为代价来改善低空间频率下的信息传输。在这里，我们证明了电子相层析成像可以在很宽的空间频谱范围内通过连续的信息传输来恢复样品的相位，并且在较低的空间频率下具有更好的传输性能，因此比传统的相衬成像更有效地进行相位恢复。我们进一步表明，该方法可以在低剂量条件下(5.7 e/a2)研究轮状病毒双层颗粒和HIV-1病毒样颗粒的冷冻水化标本，以及在大视场(1.14 × 1.14 μm)下腺病毒感染细胞中的异质物体，从而使其适合于许多生物重要结构的研究。
Cryo-electron microscopy is an essential tool for high-resolution structural studies of biological systems. This method relies on the use of phase contrast imaging at high defocus to improve information transfer at low spatial frequencies at the expense of higher spatial frequencies. Here we demonstrate that electron ptychography can recover the phase of the specimen with continuous information transfer across a wide range of the spatial frequency spectrum, with improved transfer at lower spatial frequencies, and as such is more efficient for phase recovery than conventional phase contrast imaging. We further show that the method can be used to study frozen-hydrated specimens of rotavirus double-layered particles and HIV-1 virus-like particles under low-dose conditions (5.7 e/Å2) and heterogeneous objects in an Adenovirus-infected cell over large fields of view (1.14 × 1.14 μm), thus making it suitable for studies of many biologically important structures.