Monoclonal IgM antiphosphatidylserine antibody reacts against cytoskeleton-like structures in cultured human umbilical cord endothelial cells.
Monoclonal IgM antiphosphatidylserine antibody reacts against cytoskeleton-like structures in cultured human umbilical cord endothelial cells.
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单克隆 IgM 抗磷脂酰丝氨酸抗体与培养的人脐带内皮细胞中的细胞骨架样结构发生反应。
DOI：
10.1111/j.1600-0897.1995.tb01145.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Rote,NS
中科院分区：
文献类型：
--
作者：
Lin,L;Shroyer,L;Walter,A;Lyden,TW;Ng,AK;Rote,NS
PROBLEM: It has been proposed that antibodies against phospholipid‐dependent antigens (aPLs), induce recurrent pregnancy loss and thrombosis through modulation of endothelial cell function, yet aPLs have not been conclusively shown to bind with endothelial cells.METHOD: Using indirect immunofluorescence we investigated the anti‐endothelial cell reactivity of three monoclonal antibodies that differentiate between the phospholipids cardiolipin (CL) and phosphatidylserine (PS): BA3B5C4 (CL+/PS+); 3SB9b (CL‐/PS+); and D11A4 (CL+/PS‐). Cultured umbilical cord endothelial cells were prepared without fixation or with cold acetone fixation.RESULTS: None of the aPLs reacted with endothelial cells prepared without fixation. 3SB9B reacted strongly with cytoskeletal‐like components in acetone‐fixed cells, whereas BA3B5C4 and D11A4 were unreactive. The cytoskeletal‐like binding of 3SB9b was completely blocked by a monoclonal antibody against vimentin, whereas antibodies against tubulin or actin were not inhibitory. Lipid extraction of the cells destroyed the 3SB9b reactive antigen without affecting the reactivity of anti‐vimentin.CONCLUSION: These results suggest that phospholipid‐dependent antigenic determinants are not expressed on the surface of resting endothelial cells but that a PS‐dependent antigenic determinant is associated with endothelial cell intermediate filaments.
