Detection of biological and chemical agents using differential mobility spectrometry (DMS) technology
Detection of biological and chemical agents using differential mobility spectrometry (DMS) technology
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DOI：
10.1109/jsen.2005.845515
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发表时间：
2005-08-01
影响因子：
4.3
通讯作者：
Davis, CE
中科院分区：
文献类型：
--
作者：
Krebs, MD;Zapata, AM;Davis, CE
随着国际社会对化学和生物恐怖主义的可能性日益关注，迫切需要一种能够快速、准确地检测化学和生物制剂的传感器。这种传感器需要便携、坚固、灵敏，并且具有快速的样品分析时间。我们将演示如何使用具有这些特性的微机械差分迁移谱仪 (DMS)，该谱仪可以在数秒的时间尺度内同时检测多个试剂。在这项研究中，我们证明了 DMS 检测枯草芽孢杆菌孢子的能力，枯草芽孢杆菌孢子是炭疽芽孢杆菌孢子（炭疽病的病原体）的替代品。使用热解作为样品引入方法，在将材料引入 DMS 之前挥发孢子。此外，我们使用 SDS-PAGE 检查了热解对悬浮在无菌水中的枯草芽孢杆菌孢子的影响。这些实验表明，孢子必须在 650 摄氏度或更高温度下加热 5 秒，或在 550 摄氏度下加热至少 10 秒，才能破碎成远小于 10 kDa 的颗粒，DMS 可以检测到这一点。在悬浮孢子的无菌水的背景之上可以很容易地区分几种主要的生物标志物，我们假设通过进一步优化条件可以释放更多的生物标志物。 DMS 还显示出作为化学武器制剂检测器的前景，我们已经证明了 DMS 能够检测临床相关水平的神经和疱疹制剂模拟物。
With international concern growing over the potential for chemical and biological terrorism, there-is an urgent need for a sensor that can quickly and accurately detect chemical and biological agents. Such a sensor needs to be portable, robust, and sensitive, with fast sample analysis time. We will demonstrate the use of a micromachined differential mobility spectrometer (DMS) with these characteristics that can detect multiple agents simultaneously on a time scale of seconds. In this study, we have demonstrated the ability of the DMS to detect Bacillus subtilis spores, a surrogate for Bacillus anthracis spores, the causative agent of anthrax. Pyrolysis was used as the sample introduction method to volatilize the spores before introducing material into the DMS. Additionally, we examined the effect of pyrolysis on B. subtilis spores suspended in sterile water using SDS-PAGE. These experiments showed that the spores must be heated at 650 degrees C or greater for 5 s or at 550 degrees C for at least 10 s to be fragmented into particles considerably smaller than 10 kDa, which the DMS can detect. Several major biomarkers can be easily distinguished above the background of the sterile water in which the spores are suspended, and we hypothesize that additional biomarkers could be liberated by further optimizing conditions. The DMS also has shown promise as a detector for chemical weapon agents, and we have demonstrated the ability of the DMS to detect nerve and blister agent simulants at clinically relevant levels.