Bioluminescence Tomography-Guided Radiation Therapy for Preclinical Research.
Bioluminescence Tomography-Guided Radiation Therapy for Preclinical Research.
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DOI：
10.1016/j.ijrobp.2015.11.039
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发表时间：
2016-04-01
期刊：
影响因子：
--
通讯作者：
Wong JW
中科院分区：
文献类型：
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作者：
Zhang B;Wang KK;Yu J;Eslami S;Iordachita I;Reyes J;Malek R;Tran PT;Patterson MS;Wong JW
在临床前放射研究中，基于锥形束计算机断层扫描（CBCT）引导的软组织靶定位具有挑战性。作为一种更有效的软组织靶点定位方法，我们为小动物辐射研究平台（SARRP）开发了一个在线生物发光断层成像（BLT）系统。我们证明了BLT引导的放射治疗和验证的靶向精度，基于一个新开发的重建算法。BLT系统旨在对接到SARRP上进行图像采集，并在辐射输送之前分离。设计了一个3镜系统，将受试者发出的生物发光反射到固定的CCD相机上。多光谱BLT和不完全变量截断共轭梯度法与允许区域收缩策略作为优化方案重建生物发光源分布。为了验证BLT靶向准确性，将具有高CBCT对比度的小圆柱形光源放置在体模中，并且还放置在小鼠尸体的腹部中。从BLT中恢复源的质心（CoM），并用于引导辐射输送。BLT引导靶向的准确性通过胶片进行了验证，并与CBCT引导递送进行了比较。进行了体内实验，以证明BLT定位能力的各种来源的几何形状。与CBCT定位相比，在线BLT能够以1 mm的平均精度恢复嵌入光源的CoM。胶片上显示的BLT和CBCT引导照射之间的差异与BLT和CBCT图像中显示的源定位一致。体内实验结果表明，我们的BLT系统可以潜在地应用于多个靶点和肿瘤。在线BLT/CBCT/SARRP系统为软组织靶向提供了有效的解决方案，特别是对于小的、不可触及的或原位的肿瘤模型。
In pre-clinical radiation research, it is challenging to localize soft tissue targets based on cone beam computed tomography (CBCT)-guidance. As a more effective method to localize soft tissue targets, we developed an online bioluminescence tomography (BLT) system for the small animal radiation research platform (SARRP). We demonstrated BLT-guided radiotherapy and validated targeting accuracy, based on a newly developed reconstruction algorithm. The BLT system was designed to dock onto the SARRP for image acquisition and to be detached before radiation delivery. A 3-mirror system was devised to reflect the bioluminescence emitted from the subject to a stationary CCD camera. Multispectral BLT and the incomplete variables truncated conjugate gradient method with a permissible region shrinking strategy were employed as the optimization scheme to reconstruct bioluminescent source distributions. To validate BLT targeting accuracy, a small cylindrical light source with high CBCT contrast was placed in a phantom and also in the abdomen of a mouse carcass. The center of mass (CoM) of the source was recovered from BLT and used to guide radiation delivery. The accuracy of the BLT-guided targeting was validated with films and compared with the CBCT-guided delivery. In vivo experiments were conducted to demonstrate the BLT localization capability for various source geometries. Online BLT was able to recover the CoM of the imbedded light source with an average accuracy of 1 mm compared to CBCT localization. The difference between the BLT- and CBCT-guided irradiation shown on the films was consistent with the source localization revealed in the BLT and CBCT images. The in vivo results demonstrated that our BLT system could potentially be applied for multiple targets and tumors. The online BLT/CBCT/SARRP system provides an effective solution for soft tissue targeting, particularly for small, non-palpable, or orthotopic tumor models.