Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory
Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory
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DOI：
10.1038/35078077
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发表时间：
2001-05-24
期刊：
影响因子：
64.8
通讯作者：
Tully, T
中科院分区：
文献类型：
--
作者：
Dubnau, J;Grady, L;Tully, T
手术、药理学和遗传损伤研究揭示了不同的解剖部位涉及不同形式的学习。例如，对局部脑损伤患者和啮齿动物模型系统的研究表明，海马结构参与了陈述性任务的获取，但不是其长期存储的场所（1，2）。然而，这种损伤通常是不可逆的，这限制了它们在解剖记忆获取、存储和检索的时间过程中的应用（3，4）。对蜜蜂和苍蝇的研究同样揭示了昆虫大脑的一个独特的解剖区域，蘑菇体，它特别涉及嗅觉联想学习（5，6）。我们已经使用了一种温度敏感的发动蛋白转基因，它可逆地破坏突触传递，时间尺度为分钟（7），以研究记忆形成期间持续神经活动的时间要求。在这里，我们表明，从蘑菇体神经元的突触传递是需要在记忆检索，但不是在收购或存储。我们建议，赫布过程的基础嗅觉联想学习驻留在蘑菇体树突或蘑菇体的上游，并在突触强度的变化调制蘑菇体输出在记忆检索。
Surgical, pharmacological and genetic lesion studies have revealed distinct anatomical sites involved with different forms of learning. Studies of patients with localized brain damage and work in rodent model systems, for example, have shown that the hippocampal formation participates in acquisition of declarative tasks but is not the site of their long-term storage(1,2). Such lesions are usually irreversible, however, which has limited their use for dissecting the temporal processes of acquisition, storage and retrieval of memories(3,4). Studies in bees and flies have similarly revealed a distinct anatomical region of the insect brain, the mushroom body, that is involved specifically in olfactory associative learning(5,6). We have used a temperature-sensitive dynamin transgene, which disrupts synaptic transmission reversibly and on the time-scale of minutes(7), to investigate the temporal requirements for ongoing neural activity during memory formation. Here we show that synaptic transmission from mushroom body neurons is required during memory retrieval but not during acquisition or storage. We propose that the hebbian processes underlying olfactory associative learning reside in mushroom body dendrites or upstream of the mushroom body and that the resulting alterations in synaptic strength modulate mushroom body output during memory retrieval.