A 3D, Magnetically Actuated, Aligned Collagen Fiber Hydrogel Platform Recapitulates Physical Microenvironment of Myoblasts for Enhancing Myogenesis
A 3D, Magnetically Actuated, Aligned Collagen Fiber Hydrogel Platform Recapitulates Physical Microenvironment of Myoblasts for Enhancing Myogenesis
复制标题
3D、磁驱动、对齐胶原纤维水凝胶平台重现成肌细胞的物理微环境，以增强肌生成
DOI：
10.1002/smtd.202100276
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发表时间：
2021
期刊：
影响因子：
12.4
通讯作者：
Feng Xu
中科院分区：
文献类型：
--
作者：
Nianyuan Shi;Yuhui Li;Le Chang;Guoxu Zhao;Guorui Jin;Yi Lyu;Guy M. Genin;Yufei Ma;Feng Xu
许多支持组织发育、成熟和功能的细胞反应是由细胞外基质(ECM)的结构和机械负荷引导的。由于机械刺激必须通过ECM架构传递，因此这两个因素之间的协同非常重要。然而，在体外重述这些物理微环境线索的协同作用仍然具有挑战性。为了解决这一问题，开发了一种3D磁致动胶原水凝胶平台，该平台能够组合控制ECM结构和机械刺激。通过这一平台，展示了这些因素如何协同促进C2C12成肌细胞的细胞排列和促进肌肉生成。这种促进作用在一定程度上是由Yes相关蛋白的动态变化和细胞微管网络结构驱动的。这一便捷的平台为调控细胞行为和命运、在体外生成广泛的具有生理代表性的工程化微组织以及量化其功能背后的机械生物学提供了巨大的希望。
Many cell responses that underlie the development, maturation, and function of tissues are guided by the architecture and mechanical loading of the extracellular matrix (ECM). Because mechanical stimulation must be transmitted through the ECM architecture, the synergy between these two factors is important. However, recapitulating the synergy of these physical microenvironmental cues in vitro remains challenging. To address this, a 3D magnetically actuated collagen hydrogel platform is developed that enables combined control of ECM architecture and mechanical stimulation. With this platform, it is demonstrated how these factors synergistically promote cell alignment of C2C12 myoblasts and enhance myogenesis. This promotion is driven in part by the dynamics of Yes‐associated protein and structure of cellular microtubule networks. This facile platform holds great promises for regulating cell behavior and fate, generating a broad range of engineered physiologically representative microtissues in vitro, and quantifying the mechanobiology underlying their functions.