Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d-Cycloserine in a Silkworm Infection Model
Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d-Cycloserine in a Silkworm Infection Model
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DOI：
10.1128/aac.00200-09
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发表时间：
2009-09
影响因子：
4.9
通讯作者：
K. Kurokawa;H. Hamamoto;M. Matsuo;S. Nishida;N. Yamane;B. Lee;K. Murakami;H. Maki;K. Sekimizu
中科院分区：
文献类型：
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作者：
K. Kurokawa;H. Hamamoto;M. Matsuo;S. Nishida;N. Yamane;B. Lee;K. Murakami;H. Maki;K. Sekimizu
摘要：建立家蚕幼虫感染模型，评价抗生素的治疗效果。d-环丝氨酸对金黄色葡萄球菌ddlA突变体介导的幼虫杀伤作用的50%有效剂量（ED50）显著低于亲本菌株介导的幼虫杀伤作用。其他抗生素的mic和ED50变化可以忽略不计，表明这些变化是d-环丝氨酸选择性的。因此，该模型对于在抗生素开发过程中根据其治疗效果选择所需化合物是有用的。
ABSTRACT The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of d-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.