Implications for the role of cognate interactions in in vitro human B cell activation by Staphylococcus aureus Cowan I and pokeweed mitogen.
Implications for the role of cognate interactions in in vitro human B cell activation by Staphylococcus aureus Cowan I and pokeweed mitogen.
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金黄色葡萄球菌 Cowan I 和美洲商陆有丝分裂原在体外人 B 细胞激活中同源相互作用的作用的意义。
DOI：
10.1172/jci112290
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Tsunematsu
中科院分区：
文献类型：
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作者：
N. Suzuki;T. Sakane;Y. Ueda;Y. Murakawa;T. Tsunematsu
使用多克隆激活剂金黄色葡萄球菌 Cowan I (SAC) 和商陆有丝分裂原 (PWM) 研究了人类 B 细胞触发机制。当B细胞、T细胞和单核细胞培养物经SAC或PWM刺激5 d时，B细胞可被两种丝裂原激活增殖并分泌Ig。即使当 T 细胞被 T 细胞衍生的可溶性因子取代时，SAC 刺激的 B 细胞也可以分化为 Ig 分泌细胞。相比之下，B 细胞和 T 细胞至少在培养的前 6 小时内相互作用对于 PWM 触发 B 细胞是必要的。通过 PWM 更精确地描述 B 细胞触发机制的实验表明，B 细胞触发需要 B 细胞与 T4+ 细胞而不是 T8+ 细胞的相互作用；抗 Ia 或抗 T4 抗体可以阻断这种触发；相反，抗T3或抗T8抗体不会对B细胞触发产生任何影响。然而，所有这些单克隆抗体都无法调节已被 PWM 激活的 B 细胞对 T 细胞衍生因子做出反应的能力。这些数据表明，SAC 可以直接激活 B 细胞，而 B 细胞上的 Ia 样抗原和 T4+ 细胞之间的同源相互作用对于 PWM 触发 B 细胞至关重要。此外，一旦B细胞被触发，它们就会增殖、分化并分泌Ig以响应T细胞衍生的因子； Ia 样抗原或 T 细胞分化抗原可能不参与该级联的过程。
Human B cell-triggering mechanisms were investigated using the polyclonal activators Staphylococcus aureus Cowan I (SAC) and pokeweed mitogen (PWM). When the cultures of B cells, T cells, and monocytes were stimulated for 5 d by SAC or PWM, B cells could be activated by both mitogens to proliferate and secrete Ig. Even when T cells were substituted by T cell-derived soluble factors, SAC-stimulated B cells could differentiate into Ig-secreting cells. In contrast, interactions of B and T cells for at least the first 6 h of culture were necessary for the B cell triggering by PWM. Experiments that allow a more precise delineation of the B cell-triggering mechanisms by PWM demonstrated that interactions of B cells with T4+ but not T8+ cells are required for the B cell triggering; anti-Ia or anti-T4 antibody can block this triggering; in contrast, anti-T3 or anti-T8 antibody do not exert any effects on the B cell triggering. However, all these monoclonal antibodies could not modulate the ability of B cells that had been already activated by PWM to respond to T cell-derived factors. These data suggest that SAC can directly activate B cells, while cognate interactions between Ia-like antigens on B cells and T4+ cells are essential for B cell triggering by PWM. Furthermore, once B cells are triggered, they will proliferate, differentiate, and secrete Ig in response to T cell-derived factors; Ia-like antigens or T cell differentiation antigens may not be involved in the processes in this cascade.
抗 Leu-2 和抗 Leu-3 抗体阻断人 T 淋巴细胞功能：增殖和抑制的差异抑制。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Engleman,EG;Benike,CJ;Metzler,C;Gatenby,PA;Evans,RL
通讯作者：
Evans,RL
商陆有丝分裂原刺激 T 细胞亚群增殖的调控相互作用。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Puck,JM;Rich,RR
通讯作者：
Rich,RR