Neuroprotection by resveratrol against traumatic brain injury in rats
Neuroprotection by resveratrol against traumatic brain injury in rats
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DOI：
10.1007/s11010-006-9253-0
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发表时间：
2007-01-01
影响因子：
4.3
通讯作者：
Yologlu, Saim
中科院分区：
文献类型：
--
作者：
Ates, Ozkan;Cayli, Suleyman;Yologlu, Saim
创伤性脑损伤后的氧化应激可能导致许多病理生理变化。天然存在于葡萄皮、葡萄籽和红酒中的高浓度白藜芦醇，在各种体外和体内模型中都具有显著的抗氧化特性。本研究探讨了白藜芦醇对大鼠创伤性脑损伤后氧化应激的影响。选用成年Wistar白化雄性大鼠54只，体重250 ~ 300 g。这些大鼠被分成三组。第一组为只开颅的对照组（假手术组），其余为创伤组和白藜芦醇组。白藜芦醇组和创伤组分别于创伤后立即腹腔注射100 mg/kg单剂量白藜芦醇，单剂量白藜芦醇组分别用50%乙醇溶解，2%生理盐水稀释。颅脑外伤采用减重法。然后将各组分为3个亚组，分别进行创伤后的生化分析、脑含水量和组织病理学评估。观察损伤后24小时脑含水量及损伤半球丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）、黄嘌呤氧化酶（XO）水平。伤后第14天进行定量组织病理学分析。与对照组相比，创伤导致MDA、XO、NO水平显著升高，GSH水平显著降低。白藜芦醇可显著降低MDA、XO和NO水平，增加GSH水平，并可显著减小组织病变面积。我们的研究结果表明，创伤性脑损伤后立即用白藜芦醇治疗可以减少氧化应激和损伤体积。未来涉及不同剂量和剂量-反应关系的研究可能会带来更好的结果。
Oxidative stress after traumatic brain injury may contribute to many of the pathophysiologic changes. Resveratrol, naturally present at high concentration in grape skin, seeds, and red wine, has significant antioxidant properties in a variety of in vitro and in vivo models. In this study, we investigate the effect of resveratrol on oxidative stress after traumatic brain injury in rat model.A total of 54 adult Wistar albino male rats weighing 250-300 g were used. The rats were allocated into three groups. The first group was control (sham-operated) group in which only a craniotomy was performed, the others were trauma and resveratrol groups. A 100 mg/kg single dose of resveratrol, freshly prepared by dissolving in 50% ethanol and diluted in physiological saline (2%), for resveratrol group, and 1 ml ethanol (2%) for trauma group, was administered intraperitoneally immediately after trauma. Weight-drop method was used for achieving head trauma. Then, all groups were separated into three subgroups for biochemical analysis, brain water content and histopathological assessment following trauma. Twenty-four hours after trauma brain water content and malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), xanthine oxidase (XO) levels of traumatic hemisphere were evaluated. Quantitative histopathological analysis was performed on 14th day postinjury. Trauma caused a significant increase in MDA, XO, NO levels and decrease in GSH level as compared to control group. Resveratrol administration significantly reduced MDA, XO and NO levels, increased GSH level, and also attenuated tissue lesion area. Our results indicate that treatment with resveratrol immediately after traumatic brain injury reduce oxidative stress and lesion volume. Future studies involving different doses and the dose-response relationship could promise better results.