Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase
Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase
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DOI：
10.1002/dvg.20261
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发表时间：
2007-01-01
期刊：
影响因子：
1.5
通讯作者：
Chen, Di
中科院分区：
文献类型：
--
作者：
Chen, Mo;Lichtler, Alexander C.;Chen, Di
出生后软骨的发育和生长受关键生长因子和信号分子的调节。为了充分了解这些调节因子的功能，需要建立一个诱导型和软骨细胞特异性的基因缺失系统来规避围产期致死性。在这份报告中，我们已经产生了一个转基因小鼠模型（Col 2a 1-CreER（T2）），其中Cre重组酶的表达是由软骨细胞特异性col 2a 1启动子在他莫昔芬诱导的方式驱动。为了确定Cre重组的特异性和效率，我们将Col 2a 1-CreER（T2）小鼠与Rosa 26 R报告小鼠繁殖。X-Gal染色显示Cre重组在三苯氧胺诱导的软骨组织中特异性实现。软骨细胞培养的体外实验也证明了4-羟基他莫昔芬诱导的Cre重组。这些结果表明，Col 2a 1-CreER（T2）转基因小鼠可用作诱导型和软骨细胞特异性基因缺失方法的有价值的工具。
Postnatal cartilage development and growth are regulated by key growth factors and signaling molecules. To fully understand the function of these regulators, an inducible and chondrocyte-specific gene deletion system needs to be established to circumvent the perinatal lethality. In this report, we have generated a transgenic mouse model (Col2a1-CreER(T2)) in which expression of the Cre recombinase is driven by the chondrocyte-specific col2a1 promoter in a tamoxifen-inducible manner. To determine the specificity and efficiency of the Cre recombination, we have bred Col2a1-CreER(T2) mice with Rosa26R reporter mice. The X-Gal staining showed that the Cre recombination is specifically achieved in cartilage tissues with tamoxifen-induction. In vitro experiments of chondrocyte cell culture also demonstrate the 4-hydroxy tamoxifen-induced Cre recombination. These results demonstrate that Col2a1-CreER(T2) transgenic mice can be used as a valuable tool for an inducible and chondrocyte-specific gene deletion approach.