Immunochemical and cytochemical studies of relaxin-containing cells in the guinea pig uterus.
Immunochemical and cytochemical studies of relaxin-containing cells in the guinea pig uterus.
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豚鼠子宫中含松弛素细胞的免疫化学和细胞化学研究。
DOI：
10.1002/aja.1001760308
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
R. Renegar
中科院分区：
文献类型：
--
作者：
L. Larkin;R. Renegar
用光学显微镜观察了豚鼠子宫和卵巢松弛素的免疫定位。电镜下观察同一组动物子宫内膜腺细胞（EGC）分泌颗粒的存在。那些表现出大量颗粒的EGC用蛋白A胶体金法对松弛素进行染色，或用硫代碳酰肼技术对碳水化合物进行染色。松弛素在中晚期妊娠动物的EGC中被发现，但在周期动物的卵巢或子宫中未检测到。虽然在检查的周期动物的大多数EGC中观察到细胞质颗粒，但发情期和发情前期动物的子宫中颗粒数量最多。来自发情期动物的EGC中的颗粒含有富含碳水化合物的物质，但不含松弛素。妊娠早期至中期（第15天和第30天）动物的子宫内膜腺细胞含有数量有限的颗粒，几乎所有颗粒都含有碳水化合物。在妊娠第45天，发现EGC含有许多颗粒。大多数颗粒含有松弛素;然而，大量EGC含有富含碳水化合物的颗粒。偶尔，EGC被认为包含两个群体的颗粒，这两个群体被认为是由松弛素和富含碳水化合物的颗粒。来自妊娠第60天的动物的EGC通常含有颗粒，其中大多数含有松弛素。在第60天动物的EGC中观察到富含碳水化合物的颗粒，但其直径较小，且数量远低于含松弛素的颗粒。哺乳期动物子宫内膜腺细胞很少含有颗粒。这些研究与以下假设一致：子宫是豚鼠松弛素的主要来源，松弛素在该物种的妊娠和分娩中起重要作用。这些观察结果表明，子宫内膜腺体及其产物在骑自行车的动物以及怀孕和分娩的动物的生理。
Uteri and ovaries from cycling, pregnant, and lactating guinea pigs were studied for immunolocalization of relaxin with the light microscope. Endometrial gland cells (EGC) from the same group of animals were examined in the electron microscope for the presence of secretory granules. Those EGC that exhibited high numbers of granules were stained either for relaxin with the protein A colloidal gold method or for carbohydrate with the thiocarbohydrazide technique. Relaxin was found in EGC from middle and late pregnant animals but was not detected in ovaries or uteri from cycling animals. While cytoplasmic granules were noted in most EGC from cycling animals examined, the number of granules was greatest in uteri from estrus and proestrus animals. Granules in EGC from estrus animals contained a carbohydrate-rich material but did not contain relaxin. Endometrial gland cells from animals in early to middle stages of pregnancy (days 15 and 30) contained limited numbers of granules, almost all of which contained carbohydrate. At day 45 of pregnancy, EGC containing many granules were noted. The majority of granules contained relaxin; however, a significant number of EGC contained carbohydrate-rich granules. Infrequently, EGC were noted that contained two populations of granules, and these two populations were assumed to be made up of relaxin-containing and carbohydrate-rich granules. EGC from animals on day 60 of pregnancy typically contained granules, and the majority of these contained relaxin. Carbohydrate-rich granules were observed in EGC of the day 60 animals but were smaller in diameter and were noted in much lower numbers than the relaxin-containing granules. Endometrial gland cells from lactating animals infrequently contained granules. These studies are consistent with the hypothesis that the uterus is the primary source of relaxin in the guinea pig and that relaxin plays an important role in pregnancy and parturition of this species. The observations implicate endometrial glands and their products in the physiology of the cycling animal as well as the pregnant and parturient animal.
怀孕和周期小鼠卵巢松弛素的免疫细胞化学研究。
DOI：
10.1177/33.4.2579996
发表时间：
1985
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Vaupel,MR;Sherwood,OD;Anderson,MB
通讯作者：
Anderson,MB