MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers
MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers
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DOI：
10.1016/j.neulet.2011.11.006
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发表时间：
2012-02-02
影响因子：
2.5
通讯作者：
Galimberti, Daniela
中科院分区：
文献类型：
--
作者：
Martinelli-Boneschi, Filippo;Fenoglio, Chiara;Galimberti, Daniela
鉴定新的靶点和生物标志物，如microrna，对了解多发性硬化症（MS）等疾病的发病机制非常有帮助。我们检测了19名MS患者和14名对照者外周血单个核细胞（PBMCs）中1145个microrna的表达谱，并通过全基因组mRNA谱分析和生物信息学预测工具进一步探讨了它们的功能。共有104个mirna在MS患者中被鉴定为失调：其中排名最高的2/10 （let-7g和miR-150）已在复制样本中得到验证，从而鉴定出假定的靶基因。2011爱思唯尔爱尔兰有限公司版权所有。
Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients: 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes. (C) 2011 Elsevier Ireland Ltd. All rights reserved.