Origin and evolution of Georgia 98 (GA98), a new serotype of avian infectious bronchitis virus
Origin and evolution of Georgia 98 (GA98), a new serotype of avian infectious bronchitis virus
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DOI：
10.1016/s0168-1702(01)00345-8
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发表时间：
2001-11-28
期刊：
影响因子：
5
通讯作者：
Jackwood, MW
中科院分区：
文献类型：
--
作者：
Lee, CW;Jackwood, MW
我们之前发现了GA 98。传染性支气管炎病毒（IBV）的一个新血清型。该毒株与DE 072血清型IBV在遗传学上密切相关，但抗原性上不相关。本文所我们分析了GA 98 IBV S1亚基的高变区（HVR）（从ATG起始位点开始计数的位置114-534）的421 bp序列，以进一步研究这些病毒的进化。这些病毒是在1997年至2000年间分离出来的。系统发育分析表明，来自格鲁吉亚不同地区的GA 98分离株是DE 072血清型祖先的S1基因单一导入的结果，大多数突变是非同义的，并以渐进的方式固定。HVR的进化率和突变率分别为每年2.5%和1.5%。与其他血清型相比，这种新的IBV血清型似乎进化得非常快。我们进一步确定了从北格鲁吉亚的一个选定的地区获得的7个分离株的S1基因的完整编码序列。以及病毒中和数据。GA 98似乎是由使用DE 072疫苗引起的免疫选择产生的。这一结论的原因进行了讨论。(C)2001 Elsevier Science B. V.保留所有权利。
We previously identified GA98. a new serotype of infectious bronchitis virus (IBV). which is closely related to the DE072 serotype of IBV genetically, but not antigenically. Herein. we analyzed the 421bp sequence of a hypervariable region (HVR) (position 114-534, counting from the ATG start site) of the S1 subunit of GA98 IBVs to further examine the evolution of these viruses. These viruses were isolated between the years 1997 and 2000. Phylogenetic analysis of the deduced amino acid sequence on that region indicated that GA98 isolates from different regions of Georgia were the result of a single introduction of the Sl gene of the DE072 serotype progenitor, Most of the mutations were nonsynonymous and had become fixed in a progressive manner. The evolutionary and mutation rates in the HVR was calculated as 2.5 and 1.5% per year, respectively. This new serotype of IBV appears to be evolving very fast compared with other serotypes of IBV. We further determined the complete coding sequence of the S1 gene of seven isolates obtained from one selected region in North Georgia. Together with virus neutralization data. it appears that GA98 arose from immune selection caused by DE072 vaccine use. Reasons for this conclusion are discussed. (C) 2001 Elsevier Science B.V. All rights reserved.