Hybridization-based karyotyping of mouse chromosomes: hybridization-bands.
Hybridization-based karyotyping of mouse chromosomes: hybridization-bands.
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基于杂交的小鼠染色体核型分析：杂交带。
DOI：
10.1159/000015425
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hozier,JC
中科院分区：
文献类型：
--
作者：
Liechty,MC;Carpio,CM;Aytay,S;Clase,AC;Puschus,KL;Sims,KR;Davis,LM;Hozier,JC
我们已经开发了一种方法，我们称之为杂交显带，同时确定所有染色体在小鼠中期传播。该方法使用一个单一的荧光标记的杂交探针的组合，以产生一个简单的，独特的，容易识别的杂交模式在每个染色体上。该方法上级于用于染色体鉴定的基于Giemsa或荧光的显带方法，因为杂交模式更简单且更容易鉴定，并且可以随意为每条染色体设计独特的模式。分析可以用标准的荧光显微镜进行，图像可以用普通的35毫米相机记录在胶片上，使该方法对许多研究人员有用。该方法也可以应用于任何物种，其染色体和探针可以制备。
We have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. The method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. The method is superior to Giemsa-or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique patterns can be designed at will for each chromosome. Analysis can be performed with a standard fluorescence microscope, and images can be recorded on film with an ordinary 35-mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prepared.