Transforming growth factor beta 1 gene expression during vaginal vs cutaneous surgical wound healing in the rabbit
Transforming growth factor beta 1 gene expression during vaginal vs cutaneous surgical wound healing in the rabbit
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DOI：
10.1007/s00192-012-1905-x
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发表时间：
2013-04
影响因子：
1.8
通讯作者：
Y. Abramov;E. Hirsch;V. Ilievski;R. Goldberg;S. Botros;P. Sand
中科院分区：
文献类型：
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作者：
Y. Abramov;E. Hirsch;V. Ilievski;R. Goldberg;S. Botros;P. Sand
前言和假设盆腔重建手术的结果与阴道和盆腔伤口的愈合过程密切相关。转化生长因子β 1（Transforming growth factor beta 1，TGF-β1）是皮肤组织创伤修复的主要介质。我们试图评估这一因素的表达在阴道和皮肤外科伤口repair in the rabbit.MethodsWe切除双边6毫米全厚圆形段从腹部皮肤和阴道在36新西兰白色（NZW）nulliparous雌兔。在组织创伤前、创伤当天、创伤后4、7、10、14、21、28和35天处死动物，通过实时聚合酶链反应（PCR）评估其创伤表面积和TGF-β 1基因转录。此后不久下降，在第35天达到最小值。结论TGF-β 1基因转录水平与阴道和皮肤创伤愈合率显著相关，提示TGF-β1基因转录水平参与了阴道和皮肤创伤的修复过程。
Introduction and hypothesisReconstructive pelvic surgery outcome is closely related to the vaginal and pelvic wound healing processes. Transforming growth factor beta 1 (TGF-β1) is a principal mediator of wound repair in dermal tissue. We sought to assess this factor’s expression in vaginal and dermal surgical wound repair in the rabbit.MethodsWe excised bilateral 6-mm full-thickness circular segments from the abdominal skin and vagina in 36 New Zealand White (NZW) nulliparous female rabbits. Animals were sacrificed before, on the day of, and 4, 7, 10, 14, 21, 28, and 35 days after tissue wounding, and their wounds were assessed for surface area and TGF-β1 gene transcription by real-time polymerase chain reaction (PCR).ResultsIn both the abdominal skin and vagina, TGF-β1 gene transcription increased immediately after tissue injury, reaching maximal levels on days 4–7, and decreased shortly thereafter, attaining minimal values on day 35. A significant correlation between TGF-β1 expression and the wound’s closure rate was found in both tissues.ConclusionsTGF-β1 gene transcription significantly correlates with the surgical vaginal and dermal wound closure rate, implying that this factor is involved in the process of wound repair in both tissues.