Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.
Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.
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DOI：
10.1093/nar/gkv625
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发表时间：
2015-08-18
影响因子：
14.9
通讯作者：
Doherty AJ
中科院分区：
文献类型：
--
作者：
Guilliam TA;Keen BA;Brissett NC;Doherty AJ
直到最近，DNA引物还被简单地视为一类蛋白质，它们合成启动DNA复制所必需的短RNA引物。然而，最近的研究表明，这种认为与这些不同的酶相关的有限活性的看法已经不再合理。现在可以引用许多例子来说明术语‘DNA Primase’如何仅描述这些核苷酸转移酶中的一个非常小的子集，绝大多数履行从DNA复制到损伤容忍和修复的多功能角色。本文主要介绍考古-真核普里米酶(AEP)超家族，从生命各个领域的最新特化实例来突出这些酶在功能上的多样性。AEP的广泛来源、功能和酶功能强调了它们以前的功能错误注释，并支持将这些酶重新分类为更广泛的聚合酶功能群中的一种称为初级聚合酶的类别。重要的是，以这种方式重新定位AEP更好地认识到它们在DNA新陈代谢中的更广泛作用，并鼓励发现这些酶的额外功能，除了这里强调的那些功能。
Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication. However, recent studies have shown that this perception of the limited activities associated with these diverse enzymes can no longer be justified. Numerous examples can now be cited demonstrating how the term ‘DNA primase’ only describes a very narrow subset of these nucleotidyltransferases, with the vast majority fulfilling multifunctional roles from DNA replication to damage tolerance and repair. This article focuses on the archaeo-eukaryotic primase (AEP) superfamily, drawing on recently characterized examples from all domains of life to highlight the functionally diverse pathways in which these enzymes are employed. The broad origins, functionalities and enzymatic capabilities of AEPs emphasizes their previous functional misannotation and supports the necessity for a reclassification of these enzymes under a category called primase-polymerases within the wider functional grouping of polymerases. Importantly, the repositioning of AEPs in this way better recognizes their broader roles in DNA metabolism and encourages the discovery of additional functions for these enzymes, aside from those highlighted here.
来自超嗜热古细菌硫磺菌的异二聚体引物酶在不连续模板上进行模板依赖性聚合。
影响因子：
14.9
作者：
Hu J;Guo L;Wu K;Liu B;Lang S;Huang L
通讯作者：
Huang L