IMMUNO-ENZYMATIC ASSAY OF CORTISOL USING ESCHERICHIA-COLI BETA-GALACTOSIDASE AS LABEL
IMMUNO-ENZYMATIC ASSAY OF CORTISOL USING ESCHERICHIA-COLI BETA-GALACTOSIDASE AS LABEL
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DOI：
10.1016/0022-1759(76)90167-8
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发表时间：
1976-01-01
影响因子：
2.2
通讯作者：
CELADA, F
中科院分区：
文献类型：
--
作者：
COMOGLIO, S;CELADA, F
通过使用大肠杆菌β-半乳糖苷酶作为标记物。每个酶分子中皮质醇残留的数量和游离酶与抗体结合酶的分离方法被证明是评估该测定的关键因素。每管皮质醇的灵敏度范围为100-150 pg。与其他方法的比较，即，竞争性蛋白结合分析，放射免疫分析和荧光分析。
An enzyme-assay of plasma cortisol was established by using Escherichia coli .beta.-galactosidase as a marker. The number of cortisol residues per molecule of enzyme and the method of separation of free from antibody-bound enzyme proved to be critical factors for the assessment of the assay. A sensitivity ranging from 100-150 pg of cortisol per tube was achieved. A comparison with other methods, i.e., competitive protein binding assay, radioimmunoassay and fluorimetry, is reported.