CHROMOSOME PARTITIONING IN ESCHERICHIA-COLI - NOVEL MUTANTS PRODUCING ANUCLEATE CELLS
CHROMOSOME PARTITIONING IN ESCHERICHIA-COLI - NOVEL MUTANTS PRODUCING ANUCLEATE CELLS
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DOI：
10.1128/jb.171.3.1496-1505.1989
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发表时间：
1989-03-01
影响因子：
3.2
通讯作者：
JAFFE, A
中科院分区：
文献类型：
--
作者：
HIRAGA, S;NIKI, H;JAFFE, A
为了研究细胞分裂中染色体的分配机制，我们利用新开发的技术分离了一种新型的大肠杆菌突变体，该突变体形成无核细胞。其中一个，命名为MukA1，是不致命的，并产生正常大小的无核细胞的频率为0.5%至3%的总细胞在指数增长的人口，但不产生丝状细胞。结果表明，该突变体的染色体定位不规则，不能将复制的子染色体分配到两个子细胞中，导致产生一个无核子细胞和一个有两条染色体的子细胞。mukA1突变导致多效性效应：生长缓慢，对十二烷基硫酸钠过敏，对大肠杆菌素E1蛋白耐受，以及无核细胞形成。mukA基因的克隆表明muKa 1突变是隐性的，并且mukA基因与编码外膜蛋白的tolc基因相同。
To study the chromosomal partitioning mechanism in cell division, we have isolated a novel type of Escherichia coli mutants which formed anucleate cells, by using newly developed techniques. One of them, named MukA1, is not lethal and produces normal-sized anucleate cells at a frequency of 0.5 to 3% of total cells in exponentionally growing populations but does not produce filamentous cells. Resuts suggest that the mutant is defective in the chromosome positioning at regular intracellular positions and fails frequently to partition the replicated daughter chromosomes into both daughter cells, resulting in production of one anucleate daughter cell and one with two chromosomes. The mukA1 mutation causes pleiotropic effects: slow growth, hypersensitivity to sodium dodecyl sulfate, and tolerance to colicin E1 protein, in addition to anucleate cell formation. Cloning of the mukA gene indicates that the muKa1 mutation is recessive and that he mukA gene is identical to the tolc gene coding for an outer membrane protein.