Plasma soluble tumor necrosis factor alpha receptors and leptin levels in normal-weight and obese women:: effect of adiposity and diabetes
Plasma soluble tumor necrosis factor alpha receptors and leptin levels in normal-weight and obese women:: effect of adiposity and diabetes
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DOI：
10.1530/eje.0.1460325
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发表时间：
2002-03-01
影响因子：
5.8
通讯作者：
Salas-Salvadó, J
中科院分区：
文献类型：
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作者：
Bulló, M;García-Lorda, P;Salas-Salvadó, J
目的：探讨肿瘤坏死因子α的决定因素方法：我们研究了157名肥胖的糖尿病和非糖尿病女性，分为5组：对照组-体重指数（BMI）在19和27 kg/m2之间（n = 24）;肥胖- BMI介于27和40 kg/m2之间（n = 6 3）;肥胖2型糖尿病- BMI介于27和40 kg/m2之间（n = 19），伴有糖尿病;病态肥胖- BMI > 40 kg/m2（n = 29）;病态肥胖2型糖尿病- BMI >40 kg/m2合并糖尿病（n = 22）。空腹血糖水平，血浆总甘油三酯和胆固醇，高，低和极低密度脂蛋白胆固醇测定酶法和比色法。通过ELISA测定血浆TNF α水平，通过放射免疫酶测定测定胰岛素和瘦素水平。两种可溶性TNF α（sTNF α）受体测定免疫酶法assays.Results：所有组的患者均表现出显着增加，两个sTNF α受体相对于对照组。sTNFalpha受体1（sTNFR 1）在病态肥胖糖尿病患者中高于非糖尿病患者（P = 0.003），而sTNFR 2在肥胖和病态肥胖患者中有显著差异（P = 0.036）。双变量相关分析显示，血浆sTNF α受体与BMI、体脂百分比、空腹血糖、胰岛素和瘦素显著相关。在多变量分析中，sTNF受体血浆水平均通过体脂百分比和糖尿病的存在来预测（sTNFR 1和sTNFR 2的R-2 = 0.20）。当血浆瘦素水平被添加到模型中，这种蛋白质和糖尿病的存在解释了27%的血浆sTNFR 1 levels.Conclusion的方差：糖尿病，肥胖或瘦素水平的存在是两个sTNFalpha受体的独立决定因素。肥胖患者血浆TNF α受体和瘦素水平之间的独立关联与这些蛋白质可能参与调节身体肥胖的相同途径的假设一致。
Objective: To explore the determinants of the tumor necrosis factor alpha (TNFalpha) system and their relationship with plasma leptin levels.Methods: We studied a cohort of 15 7 diabetic and non-diabetic females with a wide range of adiposity distributed into five groups: control - body mass index (BMI) between 19 and 27 kg/m(2) (n = 24); obese - BMI between 27 and 40 kg/m(2) (n = 6 3); obese type 2 diabetes mellitus - BMI between 2 7 and 40 kg/m(2) with diabetes mellitus (n = 19); morbid obese - BMI > 40 kg/m(2) (n = 29); and morbid obese type 2 diabetes mellitus - BMI >40 kg/m(2) with diabetes (n = 22). Fasting glucose levels, plasma total triglycerides and cholesterol, high-, low- and very low-density lipoprotein cholesterol were assayed by enzymatic and colorimetric methods. Plasma TNFalpha levels were measured by ELISA assay and insulin and leptin levels by radioimmunoenzymatic assays. Both soluble TNFalpha (sTNFalpha) receptors were measured by immunoenzymometric assays.Results: All groups of patients showed significant increases in both sTNFalpha receptors relative to control. sTNFalpha receptor 1 (sTNFR1) was higher in morbid obese diabetic individuals compared with their nondiabetic counterparts (P = 0.003), while sTNFR2 was significantly different between obese and morbid obese subjects (P = 0.036). Bivariate correlation analysis showed a significant relationship between both plasma sTNFalpha receptors and BMI, percentage of body fat, fasting glucose, insulin and leptin. In multivariate analysis, both sTNF receptor plasma levels were predicted by percentage of body fat and the presence of diabetes (R-2 = 0.20 for sTNFR1 and sTNFR2). When plasma leptin levels were added into the model, this protein and the presence of diabetes explained 27% of the variance of the plasma sTNFR1 levels.Conclusion: The presence of diabetes, adiposity or leptin levels are independent determinants of both sTNFalpha receptors. The independent association between plasma TNFalpha receptors and leptin levels in obese patients is consistent with the hypothesis that these proteins could be involved in the same pathway that regulates body adiposity.