Bull semen in vitro fertility after cryopreservation using egg yolk LDL:: a comparison with Optidyl®, a commercial egg yolk extender
Bull semen in vitro fertility after cryopreservation using egg yolk LDL:: a comparison with Optidyl®, a commercial egg yolk extender
复制标题
DOI：
10.1016/s0093-691x(03)00259-0
复制
发表时间：
2004-04-01
期刊：
影响因子：
2.8
通讯作者：
Anton, M
中科院分区：
文献类型：
--
作者：
Amirat, L;Tainturier, D;Anton, M
低密度脂蛋白（LDL）曾被分离并鉴定为蛋黄的冷冻保护部分。低密度脂蛋白对冻融后精子活力的影响已有报道，但尚未有研究评估低密度脂蛋白对公牛精液生育能力的影响。本研究的目的是评估低密度脂蛋白存在下冷冻保存的公牛精液的生育能力。分析了低密度脂蛋白中冷冻保存的精液的活力，并将其与含有蛋黄的商用扩展剂Optidyl(R)冷冻保存的精液进行了比较。为了评价精液的受精能力，我们采用了体外受精试验，并对顶体和质膜完整性进行了评价。活动精子的百分比。LDL（54.4%）比Optidyl（30.2%）高2倍（P < 0.05）。精后卵裂率显著高于精后卵裂率，低密度脂蛋白精子冷冻率为63.0%，而Optidyl精子冷冻率为54.8% （P < 0.05）。体外培养后囊胚率无显著差异。在两个扩展器中，顶体和质膜的完整性得以保持。综上所述，低密度脂蛋白可以保持公牛精液的质量和受精能力，并在冻融过程中提高精液的活力。(C) 2003 Elsevier Inc.版权所有。
Low-density lipoproteins (LDL) have been previously isolated and identified as the cryoprotective fraction of yolk. The effect of LDL on sperm motility after freezing-thawing has been reported, but no study has been made to assess the effect of LDL on bull semen fertility. The aim of this study was to evaluate the fertility of bull semen cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Optidyl(R), a commercial extender containing egg yolk. To evaluate the fertilizing ability of semen, we used in vitro fertilization test, whereas acrosome and plasma membrane integrity were also evaluated. The percentage of motile spermatozoa. was two fold higher after freezing in LDL than in Optidyl(R) 54.4% versus 30.2% (P < 0.05). The cleavage rate was significantly higher after fertilization, with semen frozen in LDL than with Optidyl(R) 63.0% versus 54.8% (P < 0.05). No significant difference was observed on the blastocyst rate after in vitro culture. Integrity of the acrosome and the plasma membrane were maintained in both extenders. In conclusion, LDL preserve bull semen quality and fertilizing ability, allowing also better semen motility, after the freeze-thaw process. (C) 2003 Elsevier Inc. All rights reserved.