Intracellularly grown gold nanoislands as SERS substrates for monitoring chromate, sulfate and nitrate localization sites in remediating bacteria biofilms by Raman chemical imaging.
Intracellularly grown gold nanoislands as SERS substrates for monitoring chromate, sulfate and nitrate localization sites in remediating bacteria biofilms by Raman chemical imaging.
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细胞内生长的金纳米岛作为 SERS 底物，用于通过拉曼化学成像监测修复细菌生物膜中的铬酸盐、硫酸盐和硝酸盐定位位点。
DOI：
10.1016/j.aca.2012.07.037
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发表时间：
2012
影响因子：
6.2
通讯作者：
Irudayaraj,Joseph
中科院分区：
文献类型：
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作者：
Ravindranath,SandeepP;Kadam,UlhasS;Thompson,DorotheaK;Irudayaraj,Joseph
了解生物膜基质的化学组成在不同的生物学领域，如外科手术，牙科医学，合成移植和生物修复是至关重要的。生物膜的发展，成分，活性还原位点和补救功效的知识将有助于在实施前制定有效的解决方案和评估补救方法。基于表面增强拉曼光谱（Sers）的成像是了解微生物的修复功效及其在生物膜中有机和无机化合物形成中的作用的无价工具。我们证明了第一次，铬酸盐，硫酸盐，硝酸盐和还原三价铬在土壤生物膜中的存在。此外，我们证明，Sers成像能够验证之前的研究中的铬酸盐/硫酸盐和铬酸盐/硝酸盐的相互作用在希瓦氏菌oneidensis MR-1生物膜的两个观察。此外，我们显示了一个详细的拉曼映射为基础的证据存在的铬酸盐-硫酸盐竞争进入细胞。随后，我们利用拉曼光谱研究了硝酸盐对铬酸盐还原的影响。本文中提出的研究结果是第一个报告-使用细胞内Sers底物检测细菌生物膜中的多种金属离子。使用基于金纳米岛的Sers映射基底的生物膜的这种详细表征可以扩展到研究其他金属离子和具有生物学和毒理学意义的化学物质的细胞定位及其对细菌生物膜中的还原反应的影响。
Understanding the chemical composition of biofilm matrices is vital in different fields of biology such as surgery, dental medicine, synthetic grafts and bioremediation. The knowledge of biofilm development, composition, active reduction sites and remediation efficacy will help in the development of effective solutions and evaluation of remediating approaches prior to implementation. Surface-enhanced Raman spectroscopy (SERS) based imaging is an invaluable tool to obtain an understanding of the remediating efficacy of microorganisms and its role in the formation of organic and inorganic compounds in biofilms. We demonstrate for the first time, the presence of chromate, sulfate, nitrate and reduced trivalent chromium in soil biofilms. In addition, we demonstrate that SERS imaging was able to validate two observations made by previous studies on chromate/sulfate and chromate/nitrate interactions in Shewanella oneidensis MR-1 biofilms. Additionally, we show a detailed Raman mapping based evidence of the existence of chromate–sulfate competition for cellular entry. Subsequently, we use Raman mapping to study the effect of nitrate on chromate reduction. The findings presented in this paper are among the first to report – detection of multiple metallic ions in bacterial biofilms using intracellular SERS substrates. Such a detailed characterization of biofilms using gold nanoislands based SERS mapping substrate can be extended to study cellular localization of other metallic ions and chemical species of biological and toxicological significance and their effect on reduction reactions in bacterial biofilms.