Distribution of transglutaminase family members in mouse whole body sections.
Distribution of transglutaminase family members in mouse whole body sections.
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DOI：
10.1016/j.bbrc.2015.10.001
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发表时间：
2015-11
影响因子：
3.1
通讯作者：
Hideki Tatsukawa;N. Abe;S. Ohashi;K. Hitomi
中科院分区：
文献类型：
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作者：
Hideki Tatsukawa;N. Abe;S. Ohashi;K. Hitomi
转谷氨酰胺酶（TG）是一个蛋白质家族，其成员催化各种蛋白质中谷氨酰胺和赖氨酸残基之间形成异肽键。已经鉴定出八种酶，并命名为因子XIII（FXIII）和TG 1 -7。四个主要成员的表达研究，即，FXIII、TG 1、TG 2和TG 3，与其他同工酶相比，已在相对大量的哺乳动物组织中进行。这些同工酶的结构和生化特性以及TG家族在某些组织提取物中的表达分析已有报道，但还没有同时比较分析它们在组织分布中的mRNA和蛋白表达模式。因此，我们开发了新的实验系统，用于原位杂交使用冷冻膜附着到新生小鼠的全身部分，从而获得有关的主要TG同工酶的组织分布的数据。在这项研究中，我们进行了第一次详细的比较分析的mRNA和蛋白质分布研究的TG家族成员在广泛的小鼠组织。这些数据将有助于阐明TG未知的生理和病理功能。
Transglutaminases (TGs) comprise a protein family in which the members catalyze the formation of isopeptide bonds between glutamine and lysine residues in various proteins. Eight enzymes have been identified and designated as factor XIII (FXIII) and TG1-7. Expression studies of four major members, i.e., FXIII, TG1, TG2, and TG3, have been performed in a relatively large number of mammalian tissues in comparison with those on the other isozymes. The structural and biochemical characteristics of these individual isozymes and expression analyses of TG family in some tissue extracts have been reported, but there have been no simultaneous comparative analyses of both their mRNA and protein expression patterns in tissues distributions. Thus, we developed novel experimental systems for in situ hybridization using cryofilm attached to whole body sections of neonatal mice, thereby obtaining data regarding the tissue distributions of the major TG isozymes. In this study, we performed the first detailed comparative analysis of the mRNA and protein distribution studies of TG family members in a wide range of mouse tissues. These data will be helpful for elucidating the unknown physiological and pathological functions of TGs.