Low-Intensity Pulsed Ultrasound Reduces the Inflammatory Activity of Synovitis
Low-Intensity Pulsed Ultrasound Reduces the Inflammatory Activity of Synovitis
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DOI：
10.1007/s10439-011-0408-0
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发表时间：
2011-12-01
影响因子：
3.8
通讯作者：
Tanaka, Eiji
中科院分区：
文献类型：
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作者：
Nakamura, Tatsuya;Fujihara, Shinji;Tanaka, Eiji
本研究旨在观察低强度脉冲超声(LIPUS)对炎症细胞因子刺激的滑膜细胞增殖和生长的影响，评价LIPUS治疗类风湿关节炎动物模型膝关节滑膜炎的疗效。将兔膝关节滑膜细胞系HIG-82分别加入或不加IL-1β或TNF-α培养。在细胞因子刺激4小时后，细胞接受LIPUS或假暴露。然后分析细胞增殖和生长情况。利用MRL/LPR小鼠，对LIPUS的体内抗炎作用进行了评价。促炎症细胞因子的刺激显着上调细胞增殖，而LIPUS则显着下调细胞增殖。在MRL/LPR小鼠中，与对照组相比，膝关节暴露于LIPUS导致组织学损伤显著减少。LIPUS治疗3周后，关节组织学损害明显减轻。LIPUS治疗组膝关节COX-2阳性细胞数较对照组明显减少。因此，LIPUS刺激可能是一种治疗关节炎症性疾病的药物，如滑膜炎。
The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) on the cell proliferation and growth of synovial membrane cells stimulated with inflammatory cytokines, and to evaluate the effectiveness of LIPUS treatment of synovitis in the knee joints of animal models for rheumatoid arthritis. The rabbit knee synovial membrane cell line, HIG-82, was cultured in medium with or without IL-1 beta or TNF-alpha. Four hours after stimulation with the cytokines, the cells received LIPUS or sham exposure. Cell proliferation and growth were then analyzed. Using MRL/lpr mice, the anti-inflammatory effects of LIPUS were also evaluated in vivo. Stimulation with proinflammatory cytokines significantly up-regulated cell proliferation which was significantly down-regulated by LIPUS exposure. In MRL/lpr mice, exposure of knee joints to LIPUS caused a significant reduction of histological damage compared to the control. Histological lesions were significantly reduced in the joints treated with LIPUS for 3 weeks. Cox-2-positive cells in the knee joints treated with LIPUS were markedly decreased compared to the control joints. Therefore, LIPUS stimulation may be a medical treatment for joint inflammatory diseases, such as synovitis.