The Epstein-Barr virus BFRF1 and BFLF2 proteins interact and coexpression alters their cellular localization
The Epstein-Barr virus BFRF1 and BFLF2 proteins interact and coexpression alters their cellular localization
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DOI：
10.1016/j.virol.2003.11.018
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发表时间：
2004-03-01
期刊：
影响因子：
3.7
通讯作者：
Hutt-Fletcher, LM
中科院分区：
文献类型：
--
作者：
Lake, CM;Hutt-Fletcher, LM
EB病毒（Epstein-Barr virus，EBV）的BFRF 1蛋白是最近鉴定的膜蛋白，其是α疱疹病毒UL 34基因产物的同源物。我们在这里报告，酵母双杂交筛选确定BFLF 2基因产物，一个同系物的α疱疹病毒UL 31，作为一种蛋白质，与BFRF 1相互作用。BFLF 2在哺乳动物细胞中的表达揭示了一种约2 - 8 kDa的蛋白质，与BFRF 1在非共价连接的复合物。当单独表达时，BFRF 1蛋白被发现在细胞质和核周区域。BFLF 2被发现弥漫在细胞核中的BFRF 1的情况下，但BFRF 1和BFLF 2的共表达导致在核边缘的两种蛋白质的共定位。这些数据概括了BFRF 1和BFLF 2的α疱疹病毒同源物的行为，并表明功能以及结构和位置同源性可能是保守的。(C)2004年爱思唯尔公司All rights reserved.
The BFRF1 protein of Epstein-Barr virus (EBV) is a recently identified membrane protein that is the homolog of the alphaherpesvirus UL34 gene product. We report here that a yeast two-hybrid screen identified the BFLF2 gene product, a homolog of alphaherpesvirus UL31, as a protein that interacts with BFRF1. Expression of BFLF2 in mammalian cells revealed a protein of approximately 2 8 kDa that associated with BFRF1 in a noncovalently linked complex. When expressed alone, the BFRF1 protein was found in the cytoplasm and perinuclear region. BFLF2 was found diffusely in the nucleus in the absence of BFRF1, but coexpression of BFRF1 and BFLF2 resulted in colocalization of the two proteins at the nuclear rim. These data recapitulate the behavior of the alphaherpesvirus homologs of BFRF1 and BFLF2 and suggest that functional as well as structural and positional homology may be conserved. (C) 2004 Elsevier Inc. All rights reserved.