Translocation of phospholipid/Ca2+-dependent protein kinase in B-lymphocytes activated by phorbol ester or cross-linking of membrane immunoglobulin.
Translocation of phospholipid/Ca2+-dependent protein kinase in B-lymphocytes activated by phorbol ester or cross-linking of membrane immunoglobulin.
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佛波酯或膜免疫球蛋白交联激活 B 淋巴细胞中磷脂/Ca2 依赖性蛋白激酶的易位。
DOI：
10.1042/bj2330145
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Galbraith,RM
中科院分区：
文献类型：
--
作者：
Nel,AE;Wooten,MW;Landreth,GE;Goldschmidt-Clermont,PJ;Stevenson,HC;Miller,PJ;Galbraith,RM
用佛波酯或抗免疫球蛋白刺激外周血B淋巴细胞，表明磷脂/Ca 2+依赖性蛋白激酶（C-激酶）活性从细胞质到膜组分的细胞内易位。这种现象，这是剂量和时间依赖性，被发现在正常和慢性淋巴细胞白血病B细胞。这表明C-激酶依赖性蛋白磷酸化可能与膜受体占据有关，因此可能在B淋巴细胞反应中很重要。
Stimulation of peripheral-blood B-lymphocytes with phorbol ester or anti-immunoglobulin demonstrated intracellular translocation of phospholipid/Ca2+-dependent protein kinase (C-kinase) activity from cytosol to membrane fractions. This phenomenon, which was dose- and time-dependent, was found in both normal and chronic-lymphocytic-leukemia B-cells. This suggests that C-kinase-dependent protein phosphorylation may be related to membrane receptor occupation and may therefore be important in B-lymphocyte responses.