PSEUDOMONAS-STUTZERI AND RELATED SPECIES UNDERGO NATURAL TRANSFORMATION
PSEUDOMONAS-STUTZERI AND RELATED SPECIES UNDERGO NATURAL TRANSFORMATION
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DOI：
10.1128/jb.153.1.93-99.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
INGRAHAM, JL
中科院分区：
文献类型：
--
作者：
CARLSON, CA;PIERSON, LS;INGRAHAM, JL
斯氏毕赤酵母的细胞是由同源染色体DNA自然转化的;它们不需要化学处理就能成为感受态。这种经历自然转化的能力由密切相关的物种门多萨假单胞菌、产碱假单胞菌和假产碱假单胞菌共享，但在铜绿假单胞菌、P. perfectomarinus、P. putida、P. fluorescens或P. pastingae的菌株中检测不到。施氏毕赤酵母可以在平板上或液体培养基中转化。只有双链染色体DNA是有效的;单链DNA和质粒DNA是无效的。大于10千碱基对的DNA片段比较小的片段更有效。转化频率与1 ng/ml至1 μ g/ml的DNA浓度成比例;较高的浓度是饱和的。最大频率，约10-4转化体/受体细胞，获得与细胞从培养物在早期稳定生长期。已经转化了多种染色体突变，包括营养缺陷型突变和抗生素抗性突变。目前尚未发现斯氏疟原虫中其他的遗传交换系统;转化为这种代谢多样性生物的遗传分析提供了一种手段。
Cells of P. stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation is shared by the closely related species P. mendocina, P. alcaligenes and P. pseudoalcaligenes, but was not detectable in strains of P. aeruginosa, P. perfectomarinus, P. putida, P. fluorescens or P. syringae. P. stutzeri could be transformed on plates or in liquid medium. Only double-stranded chromosomal DNA was effective; single-stranded DNA and plasmid DNA were not. DNA fragments larger than 10 kilobase pairs were more effective than smaller fragments. The transformation frequency was proportional to DNA concentration from 1 ng/ml to 1 .mu.g/ml; higher concentrations were saturating. The maximum frequency, about 10-4 transformants/recipient cell, was obtained with cells from a culture in the early stationary growth phase. A variety of chromosomal mutations have been transformed, including mutations to auxotrophy and to antibiotic resistance. Other systems for genetic exchange in P. stutzeri have not yet been found; transformation offers a means for the genetic analysis of this metabolically versatile organism.