Sulfatides are associated with neointimal thickening after vascular injury
Sulfatides are associated with neointimal thickening after vascular injury
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DOI：
10.1016/j.atherosclerosis.2010.01.033
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发表时间：
2010-07-01
期刊：
影响因子：
5.3
通讯作者：
Node, K.
中科院分区：
文献类型：
--
作者：
Inoue, T.;Taguchi, I.;Node, K.
背景：已知硫酸脂是P-选择素的天然配体。通过P-选择素和Mac-1（CD 11b/CD 18）之间的相互作用，血小板-白细胞相互作用在血管损伤后新生内膜增厚的机制中起着重要作用，如支架后再狭窄。方法：对21例冠状动脉支架植入术患者的冠状静脉窦和外周血标本进行连续检测，检测血清硫苷脂水平、血小板表面P-选择素表达和中性粒细胞表面活化的Mac-1。跨心梯度（冠状窦减去外周血）的硫苷脂水平在15分钟时显著增加（-1.47 +/- 2.87至0.59 +/- 1.44 nmol/ml，p < 0.001）。支架植入后15 min血小板表面P-选择素表达跨心脏梯度的最大反应（R = 0.55，p < 0.01），48 h时中性粒细胞表面激活的Mac-1的表达（R = 0.59，p < 0.01），均与15 min时血清硫苷脂水平呈正相关。血管造影晚期管腔丢失与trans-lung相关。15分钟时的硫苷脂水平的心脏梯度（R = 0.48，p < 0.05），15 min时血小板P-选择素表达（R = 0.42，p < 0.05）和活化的中性粒细胞Mac-1表达在48 h（R = 0.46，p < 0.05），但与其他采样点的值无关。硫酸脂可能在血管损伤的炎症反应和新生内膜增厚的发展中发挥生理作用。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Background: Sulfatides are known to be a native ligand of P-selectin. Platelet-leukocyte interaction via the cross-talk between P-selectin and Mac-1 (CD11b/CD18) plays an important role in the mechanism of neointimal thickening after vascular injury such as that seen in post-stent restenosis. However, the roles of sulfatides on restenosis have not been elucidated.Methods: Serum sulfatide levels, P-selectin expression on the surface of platelets, and activated Mac-1 on the surface of neutrophils were serially measured using both coronary sinus and peripheral blood samples in 21 patients who underwent coronary stent implantation.Results: The trans-cardiac gradient (coronary sinus minus peripheral blood) of the sulfatide levels significantly increased at 15 min (-1.47 +/- 2.87 to 0.59 +/- 1.44 nmol/ml, p < 0.001), compared to baseline levels. The maximum response of the trans-cardiac gradient of P-selectin expression on the surface of platelets at 15 min after stent implantation (R = 0.55, p < 0.01), and that of activated Mac-1 on the surface of neutrophils at 48 h (R = 0.59, p < 0.01), were both positively correlated with that of serum sulfatide levels at 15 min. The angiographic late lumen loss was correlated with the trans-cardiac gradient of sulfatide levels at 15 min (R = 0.48, p < 0.05), platelet P-selectin expression at 15 min (R = 0.42, p < 0.05) and activated neutrophil Mac-1 expression at 48 h (R = 0.46, p < 0.05), but not with values at other sampling points.Conclusions: Sulfatides may play a physiological role on inflammation in vascular injury and the development of neointimal thickening. (C) 2010 Elsevier Ireland Ltd. All rights reserved.