Antiviral activity of lactoferrin towards naked viruses
Antiviral activity of lactoferrin towards naked viruses
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DOI：
10.1023/b:biom.0000027708.27142.bc
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发表时间：
2004-06
期刊：
影响因子：
3.5
通讯作者：
L. Seganti;A. D. Di Biase;M. Marchetti;Agostina Pietrantoni;A. Tinari;F. Superti
中科院分区：
文献类型：
--
作者：
L. Seganti;A. D. Di Biase;M. Marchetti;Agostina Pietrantoni;A. Tinari;F. Superti
众所周知，乳铁蛋白（Lf）是针对多种有包膜和裸病毒（如轮状病毒、肠道病毒和腺病毒）的有效抑制剂。Lf对胰蛋白酶消化具有抗性，母乳喂养的婴儿排出高水平的粪便Lf，因此其对胃肠道中病毒复制的影响引起了极大的兴趣。在这份报告中，我们分析了这种蛋白质在三种病毒模型中的抗病毒作用机制，尽管它们代表不同的基因组和复制策略，但它们都具有感染肠道的能力。关于抗轮状病毒的作用机制，来自牛乳的Lf（BLf）具有双重作用，通过与病毒颗粒结合来防止病毒附着于肠细胞，并抑制后吸附步骤。BLf对脊髓灰质炎病毒的作用是由于对早期感染步骤的干扰，但当BLf分子被Zn+2离子饱和时，它也能够在病毒吸附阶段后抑制病毒复制。BLf的抗腺病毒作用通过竞争常见的糖胺聚糖受体和与病毒结构多肽的特异性相互作用发生在病毒附着于细胞膜上。总之，这些发现提供了进一步的证据，表明Lf是寻找抗病毒性肠道疾病的天然药物的优秀候选者，因为它主要通过特异性结合细胞受体和/或病毒颗粒来阻碍吸附和内化到细胞中。
It is well known that lactoferrin (Lf) is a potent inhibitor towards several enveloped and naked viruses, such as rotavirus, enterovirus and adenovirus. Lf is resistant to tryptic digestion and breast-fed infants excrete high levels of faecal Lf, so that its effect on viruses replicating in the gastrointestinal tract is of great interest. In this report, we analysed the mechanism of the antiviral action of this protein in three viral models which, despite representing different genoma and replication strategies, share the ability to infect the gut. Concerning the mechanism of action against rotavirus, Lf from bovine milk (BLf) possesses a dual role, preventing virus attachment to intestinal cells by binding to viral particles, and inhibiting a post adsorption step. The BLf effect towards poliovirus is due to the interference with an early infection step but, when the BLf molecule is saturated with Zn+2ions, it is also capable of inhibiting viral replication after the viral adsorption phase. The anti-adenovirus action of BLf takes place on virus attachment to cell membranes through competition for common glycosaminoglycan receptors and a specific interaction with viral structural polypeptides. Taken together, these findings provide further evidence that Lf is an excellent candidate in the search of natural agents against viral enteric diseases, as it mainly acts by hindering adsorption and internalisation into cells through specific binding to cell receptors and/or viral particles.