A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper)
A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper)
复制标题
烟碱乙酰胆碱受体突变赋予褐飞虱（褐飞虱）对吡虫啉的靶点抗性
DOI：
10.1073/pnas.0502901102
复制
发表时间：
2005-06-14
影响因子：
11.1
通讯作者：
Millar, NS
中科院分区：
文献类型：
--
作者：
Liu, ZW;Williamson, MS;Millar, NS
新烟碱类化合物，如吡虫啉，是具有强效杀虫活性的烟碱乙酰胆碱受体（nAChR）激动剂。自20世纪90年代初引入以来，吡虫啉已成为作物保护和动物健康应用中使用最广泛的杀虫剂之一。与其他类别的杀虫剂一样，对类烟碱的抗药性是一个重大威胁，已在几种害虫物种中发现，包括亚洲许多地区的主要水稻害虫褐飞虱。在这项研究中，放射性配体结合实验已经进行了与整体膜制备的吡虫啉敏感和抗性菌株的N。lugens。结果显示，[H-3]吡虫啉与敏感品系的特异性结合水平远高于与抗性品系的特异性结合水平（分别为16.7 +/- 1.0和0.34 +/- 0.21 fmol/mg蛋白质）。为了了解吡虫啉抗性的分子基础，从烟草中克隆了5个nAChR亚基（NI α 1-NI α 4和NI β 1）。lugens。比较nAChR亚基基因的吡虫啉敏感和抗性人群已经确定了一个单一的点突变在一个保守的位置（Y151 S）在两个nAChR亚基，Mal和MO。等位基因特异性PCR结果表明，Y151 S点突变频率与吡虫啉抗性水平之间存在很强的相关性。通过表达含有N. lugens α和大鼠β 2亚基，获得的证据表明突变Y151 S是导致特异性[H-3]吡虫啉结合显著降低的原因。本研究为靶标对烟碱类杀虫剂产生抗性提供了直接证据。
Neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor (nAChR) agonists with potent insecticidal activity. Since its introduction in the early 1990s, imidacloprid has become one of the most extensively used insecticides for both crop protection and animal health applications. As with other classes of insecticides, resistance to neonicotinoids is a significant threat and has been identified in several pest species, including the brown planthopper, Nilaparvata lugens, a major rice pest in many parts of Asia. In this study, radioligand binding experiments have been conducted with whole-body membranes prepared from imidacloprid-susceptible and imidacloprid-resistant strains of N. lugens. The results reveal a much higher level of [H-3]imidacloprid-specific binding to the susceptible strain than to the resistant strain (16.7 +/- 1.0 and 0.34 +/- 0.21 fmol/mg of protein, respectively). With the aim of understanding the molecular basis of imidacloprid resistance, five nAChR subunits (NI alpha 1-NI alpha 4 and NI beta 1) have been cloned from N. lugens. A comparison of nAChR subunit genes from imidacloprid-sensitive and imidacloprid-resistant populations has identified a single point mutation at a conserved position (Y151S) in two nAChR subunits, Mal and MO. A strong correlation between the frequency of the Y151S point mutation and the level of resistance to imidacloprid has been demonstrated by allele-specific PCR. By expression of hybrid nAChRs containing N. lugens alpha and rat beta 2 subunits, evidence was obtained that demonstrates that mutation Y151S is responsible for a substantial reduction in specific [H-3]imidacloprid binding. This study provides direct evidence for the occurrence of target-site resistance to a neonicotinoid insecticide.