Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene.
Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene.
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利什曼原虫主染色体 3 包含两个由 tRNA 基因分隔的长聚合多顺反子基因簇。
DOI：
10.1093/nar/gkg469
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发表时间：
2003
影响因子：
14.9
通讯作者：
Rickell,Er
中科院分区：
文献类型：
--
作者：
Worthey,EA;Martinez-Calvillo,Santiago;Schnaufer,Achim;Aggarwal,Gautam;Cawthra,Jason;Fazelinia,Gholam;Fong,Chris;Fu,Guoliang;Hassebrock,Melissa;Hixson,Greg;Ivens,AlasdairC;Kiser,Patti;Marsolini,Felicia;Rickel,Erika;Rickell,Er
利什曼原虫寄生虫（锥虫科动质体目）会引起一系列人类疾病，从无症状到致命。约 33.6 Mb 的基因组分布在 36 个染色体对中，大小范围为约 0.3 至 2.8 Mb。大型利什曼原虫弗里德林 1 号染色体的完整核苷酸序列揭示了 79 个蛋白质编码基因，它们组织成两个不同的多顺反子基因簇，mRNA 向端粒转录。我们在此报告了 3 号染色体的完整核苷酸序列（384 518 bp），并通过分析揭示了 95 个假定的蛋白质编码 ORF。 ORF 主要组织成两个大的聚合多顺反子基因簇（即从端粒转录）。此外，左端的单个基因向端粒方向发散转录，tRNA 基因将两个趋同的基因簇分开。已鉴定出许多基因，包括代谢酶、激酶、转运蛋白、核糖体蛋白、剪接体成分、解旋酶、RNA 结合蛋白和 DNA 引物酶亚基的基因。
Leishmaniaparasites (order Kinetoplastida, family Trypanosomatidae) cause a spectrum of human diseases ranging from asymptomatic to lethal. The ∼33.6 Mb genome is distributed among 36 chromosome pairs that range in size from ∼0.3 to 2.8 Mb. The complete nucleotide sequence ofLeishmania majorFriedlin chromosome 1 revealed 79 protein‐coding genes organized into two divergent polycistronic gene clusters with the mRNAs transcribed towards the telomeres. We report here the complete nucleotide sequence of chromosome 3 (384 518 bp) and an analysis revealing 95 putative protein‐coding ORFs. The ORFs are primarily organized into two large convergent polycistronic gene clusters (i.e. transcribed from the telomeres). In addition, a single gene at the left end is transcribed divergently towards the telomere, and a tRNA gene separates the two convergent gene clusters. Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA‐binding protein and a DNA primase subunit.