ALZHEIMERS-DISEASE - INITIAL REPORT OF THE PURIFICATION AND CHARACTERIZATION OF A NOVEL CEREBROVASCULAR AMYLOID PROTEIN
ALZHEIMERS-DISEASE - INITIAL REPORT OF THE PURIFICATION AND CHARACTERIZATION OF A NOVEL CEREBROVASCULAR AMYLOID PROTEIN
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DOI：
10.1016/s0006-291x(84)80190-4
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
WONG, CW
中科院分区：
文献类型：
--
作者：
GLENNER, GG;WONG, CW
一种纯化的蛋白质，其来源于扭曲的β-用SephadexG-100柱层析法和高效液相色谱法分离与阿尔茨海默病相关的脑血管淀粉样变性中的折叠片原纤维，SephadexG-100柱层析法使用1 N乙酸中的5 M盐酸胍进行分离。氨基酸序列分析和计算机检索表明，该蛋白与迄今为止测序的任何蛋白都没有同源性。这种蛋白质可能来源于一种独特的血清前体，这可能为阿尔茨海默病提供诊断测试和了解其在人类中的发病机制的手段。
A purified protein derived from the twisted .beta.-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer''s disease was isolated by Sephadex G-100 column chromatography with 5 M guanidine-HCl in 1 N acetic acid and by high performance liquid chromatography. Amino acid sequence analysis and a computer search reveals this protein has no homology with any protein sequenced thus far. This protein may be derived from a unique serum precursor which may provide a diagnostic test for Alzheimer''s disease and a means to understand its pathogenesis in man.