SYSTEMIC BLOOD PLASMA CCL5 AND CXCL6: POTENTIAL BIOMARKERS FOR HUMAN LUMBAR DISC DEGENERATION
SYSTEMIC BLOOD PLASMA CCL5 AND CXCL6: POTENTIAL BIOMARKERS FOR HUMAN LUMBAR DISC DEGENERATION
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DOI：
10.22203/ecm.v031a01
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Samartzis, D.
中科院分区：
文献类型：
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作者：
Grad, S.;Bow, C.;Samartzis, D.
磁共振成像(MRI)显示腰椎间盘退变的严重程度与下腰痛有关。致炎趋化因子CCL5和CXCL6是由诱导退变的椎间盘释放的，CCL5与椎间盘源性背痛有关。采用病例对照研究方法，以香港椎间盘退变人群为基础，研究腰椎间盘退变患者的CCL5和CXCL6水平是否高于非退变患者。入选受试者80例，其中无退变组40例(对照组，DDD评分0分)，中重度退变组40例(退变组，DDD评分=5分)。受试者在年龄、性别、体重指数和工作量方面进行匹配。从每个个体获取血浆样本，并测定CCL5和CXCL6水平。腰间盘移位和颈椎间盘改变的继发性表型也被评估。腰椎间盘退变组的CCl_5浓度(平均值：19.8 ng/mL)显著高于对照组(平均值：12.8 ng/mL)(p=0.015)。退化组CXCL6水平(平均值：56.9pg/mL)高于对照组(平均值：43.4pg/mL)(p=0.010)。退变组中CCl_5水平有升高的趋势，并伴有椎间盘移位(p=0.073)。颈椎间盘退变与趋化因子水平升高无关(p>；0.05)。这是第一项注意到全身性CCL5和CXCL6升高与中度/重度腰椎间盘退变相关的研究，进一步证实了对疼痛椎间盘的组织研究。这些趋化因子可能是诊断和监测椎间盘退变的全身性生物标志物。
Lumbar disc degeneration severity on magnetic resonance imaging (MRI) is associated with low back pain. Proinflammatory chemokines CCL5 and CXCL6 are released by induced degenerative discs, and CCL5 has been associated with discogenic back pain. A case-control study was performed, based on the Hong Kong Disc Degeneration Population-Based Cohort of Southern Chinese, to investigate if systemic levels of CCL5 and CXCL6 were elevated in subjects with disc degeneration compared to non-degenerated individuals. Eighty subjects were selected, 40 with no disc degeneration (control group; DDD score 0) and 40 with moderate/severe disc degeneration (disc degeneration group; DDD score >= 5) as noted on MRI. Subjects were matched for age, sex, body mass index and workload. Blood plasma samples were obtained from each individual, and levels of CCL5 and CXCL6 were measured. Secondary phenotypes of lumbar disc displacement and cervical disc changes were also assessed. CCL5 concentrations were significantly increased in the disc degeneration (mean: 19.8 ng/mL) compared to the control group (mean: 12.8 ng/mL) (p = 0.015). The degeneration group demonstrated higher levels of CXCL6 (mean: 56.9 pg/mL) compared to the control group (mean: 43.4 pg/mL) (p = 0.010). There was a trend towards elevated CCL5 levels with disc displacement in the degeneration group (p = 0.073). Cervical disc degeneration was not associated with elevated chemokine levels (p > 0.05). This is the first study to note that elevated systemic CCL5 and CXCL6 were associated with moderate/severe lumbar disc degeneration, further corroborating tissue studies of painful discs. These chemokines may be systemic biomarkers for the diagnosis and monitoring of disc degeneration.