The quorum-sensing system AvsR-AvsI regulates both long-chain and short-chain acyl-homoserine lactones in Agrobacterium vitis E26
The quorum-sensing system AvsR-AvsI regulates both long-chain and short-chain acyl-homoserine lactones in Agrobacterium vitis E26
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DOI：
10.1007/s10482-007-9201-3
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
Yuan-hong Wang;Li-qun Zhang;Jinyun Li;Jian-hui Wang;Hui‐min Wang
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文献类型：
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作者：
Yuan-hong Wang;Li-qun Zhang;Jinyun Li;Jian-hui Wang;Hui‐min Wang
葡萄农杆菌E26是一株从葡萄冠瘿中分离出来的非致病菌。本文报道了菌株E26中luxR-luxI型群体感应系统的鉴定。该系统参与烟草超敏反应的诱导，但不参与烟草的生物防治活性。推导出的AvsI（E26）和AvsR（E26）与葡萄球菌F2/5菌株的AvsI（F2/5）和AvsR（F2/5）的相似性最大。AvsI（E26）突变体消除了长链和短链酰基同丝氨酸内酯信号的产生以及引起烟草过敏反应的能力。avsI （E26）和avsR （E26）基因的互补使失去的表型恢复到野生型E26的水平。盆栽试验中，宽型E26与群体感应阴性突变体对葡萄冠瘿病的防效无显著差异。
Agrobacterium vitis strain E26 is a nonpathogenic bacterium isolated from grape crown gall. In this study, the identification of a luxR-luxI type quorum-sensing system in strain E26 is reported. This system is involved in the induction of hypersensitive response on tobacco, but not its biocontrol activity. The deduced components AvsI(E26) and AvsR(E26) show the greatest similarity to AvsI(F2/5) and AvsR(F2/5), respectively from A. vitis strain F2/5. The mutant in AvsI(E26) abolished the production of both long-chain and short-chain acyl-homoserine lactones signals as well as the ability to cause hypersensitive response on tobacco. Complementaion of avsI (E26) and avsR (E26) genes restored the lost phenotypes to the level of wild type E26. In pot trial, no significant difference on biocontrol efficiency against grapevine crown gall was found between the wide type E26 and its quorum sensing negative mutants.