THE DYNAMICS OF THE STEROIDOGENIC RESPONSE OF PERIFUSED-XENOPUS OVARIAN EXPLANTS TO GONADOTROPINS
THE DYNAMICS OF THE STEROIDOGENIC RESPONSE OF PERIFUSED-XENOPUS OVARIAN EXPLANTS TO GONADOTROPINS
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DOI：
10.1016/0016-6480(88)90304-8
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发表时间：
1988-07-01
影响因子：
2.7
通讯作者：
JOLY, J
中科院分区：
文献类型：
--
作者：
ELZEIN, G;BOUJARD, D;JOLY, J
在灌流系统中，研究了在LH刺激的各种条件下爪蟾卵泡成熟前和成熟过程中的类固醇释放。15 μ g LH的急性刺激诱导雄激素（睾酮和雄烯二酮）水平增加10倍，其在4小时后达到最大值，然后缓慢降低，直到第25小时。每2或4小时重复刺激后，在前8或10小时内雄激素增加相同，然后尽管注射了新的LH，但分泌量缓慢下降。只有在至少两次刺激（8小时）后才能看到孕酮分泌的显著增加。在最初的5小时内，雌二醇分泌缓慢增加到中等水平，然后无论刺激如何都保持稳定。在连续刺激（LH 0.5 μ g/ml）期间，雄激素水平在4小时后达到初始最大值，然后以2小时的周期波动。向培养基中添加茶碱增强了这些波动。12小时后，当孕酮增加时，雄激素分泌减少，达到基础水平，没有波动。生发泡破裂仅发生在经过适当刺激以在所需时间内分泌雄激素和孕激素的卵泡中。
Steroid release before and during maturation was studied in Xenopus ovarian follicles under various conditions of stimulation of LH in a perifusion system. Acute stimulation by 15 .mu.g of LH induces a 10-fold increase in the androgen (testosterone and androstenedione) level which reaches a maximum 4 hr later and then slowly decreases until the 25th hour. Repeated stimulations every 2 or 4 hr are followed by the same androgen increase during the first 8 or 10 hr and then by a slow decrease in the secretion despite new LH injections. A significant increase in progesterone secretion is seen only after at least two stimulations (8 hr). Estradiol secretion slowly increases to a moderate level during the first 5 hr and then remains stable whatever the stimulation. During continuous stimulation (LH 0.5 .mu.g/ml) androgen levels reach an initial maximum after 4 hr and then fluctuate with a 2-hr period. Addition of theophyllin to the medium enhances these fluctuations. After 12 hr when the progesterone has increased, androgen secretion diminishes to reach a basal level without fluctuations. Germinal vesicle breakdown occurs only in follicles that have been appropriately stimulated to secrete androgens and progesterone during the required time.