Changes of arbuscular mycorrhizal traits and community structure with respect to soil salinity in a coastal reclamation land
Changes of arbuscular mycorrhizal traits and community structure with respect to soil salinity in a coastal reclamation land
复制标题
DOI：
10.1016/j.soilbio.2014.01.017
复制
发表时间：
2014-05-01
影响因子：
9.7
通讯作者：
Sa, Tongmin
中科院分区：
文献类型：
--
作者：
Krishnamoorthy, Ramasamy;Kim, Kiyoon;Sa, Tongmin
全面了解土壤盐分与丛枝菌根真菌（AMF）之间的关系对于深入了解盐胁迫条件下生态系统的功能至关重要。本研究的目的是确定土壤盐分对AMF根定殖，孢子计数，球囊霉素相关的土壤蛋白（GRSP）和群落结构的影响，在韩国新万金复垦土地。土壤样品的收集和分组为5个不同的盐类土壤饱和提取物（ECSE）的电导率的基础上。测定了不同盐度条件下的菌根定殖率、孢子数和GASP。通过孢子形态学、末端限制性片段长度多态性（T-RFLP）和变性梯度凝胶电泳（DGGE）三种互补方法研究了AMF群落结构。结果表明，土壤盐分对根系定殖率（P < 0.01）、孢子数（P < 0.01）和GRSP（P < 0.01）有负影响。孢子形态和T-RFLP数据显示AMF属球囊霉属在新万金复垦地占优势。T-RFLP和DGGE分析表明，非盐渍化土壤（ECse < 2 dS/m）和极端盐渍化土壤（ECse > 16 dS/m）的物种多样性指数有显著差异，且只有在ECse < 8 dS/m的土壤中，才会出现球囊霉的优势种。然而，核糖体型的摩西球囊霉和球囊霉增殖普遍存在于所有的盐类。结合孢子形态学、T-RFLP和DGGE分析，我们可以看出AMF群落在不同盐类中有明显的影响。本研究的结果加深了我们对AMF活性和不同盐类中的优势种的了解，并将大大扩展我们对复垦土地AMF多样性的认识。(C)2014爱思唯尔有限公司版权所有。
A comprehensive knowledge on the relationship between soil salinity and arbuscular mycorrhizal fungi (AMF) is vital for a deeper understanding of ecosystem functioning under salt stress conditions. The objective of this study was to determine the effects of soil salinity on AMF root colonization, spore count, glomalin related soil protein (GRSP) and community structure in Saemangeum reclaimed land, South Korea. Soil samples were collected and grouped into five distinct salt classes based on the electrical conductivity of soil saturation extracts (ECse). Mycorrhizal root colonization, spore count and GASP were measured under different salinity levels. AMF community structure was studied through three complementary methods; spore morphology, terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Results revealed that root colonization (P < 0.01), spore count (P < 0.01) and GRSP (P < 0.01) were affected negatively by soil salinity. Spore morphology and T-RFLP data showed predominance of AMF genus Glomus in Saemangeum reclaimed land. T-RFLP and DGGE analysis revealed significant changes in diversity indices between non (ECse < 2 dS/m) and extremely (ECse > 16 dS/m) saline soil and confirmed dominance of Glomus caledonium only in soils with ECse < 8 dS/m. However, ribotypes of Glomus mosseae and Glomus proliferum were ubiquitous in all salt classes. Combining spore morphology, T-RFLP and DGGE analysis, we could show a pronounced effect in AMF community across salt classes. The result of this study improve our understanding on AMF activity and dominant species present in different salt classes and will substantially expand our knowledge on AMF diversity in reclaimed lands. (C) 2014 Elsevier Ltd. All rights reserved.