Effect of moxa smoke produced during combustion of Aiye (Folium Artemisiae Argyi) on behavioral changes in mice inhaling the smoke.
Effect of moxa smoke produced during combustion of Aiye (Folium Artemisiae Argyi) on behavioral changes in mice inhaling the smoke.
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DOI：
10.1016/s0254-6272(17)30019-5
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Jia Yang;Xiaojun Zheng;R. Jin;Li Han;Lue Ha;Jun Li;Lei Wang;Ping Liu;Zhusheng Chu;Chang Huang;Hon-Chou Chang;L. Lao;Baixiao Zhao
中科院分区：
文献类型：
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作者：
Jia Yang;Xiaojun Zheng;R. Jin;Li Han;Lue Ha;Jun Li;Lei Wang;Ping Liu;Zhusheng Chu;Chang Huang;Hon-Chou Chang;L. Lao;Baixiao Zhao
目的评价中药艾叶燃烧产物吸入的效果方法昆明种小鼠40只（一半男性）被随机分配（n= 10/组）与对照组（C）或三艾草烟浓度组（%不透明度）之一：低（L1; 0.4%）、中（M1; 2%）和高（H1; 15%）。后3组小鼠在动态气体暴露柜中暴露于艾草烟中，每天20 min，连续7 d。对照组小鼠置于同一柜内，不做任何干预。对于睡眠实验，将另外50只小鼠分成5组，每组10只小鼠：盐水注射对照组、L1 +戊巴比妥钠（PS）注射组、M1 +PS组、H1（15%）+ PS组和阳性对照组（10 mg/kg，氯丙嗪，p.o.）。记录小鼠的体重、一般活动、自主活动、转棒能力、睡眠时间和阈下剂量戊巴比妥钠诱导的睡眠率，并采用顶空气相色谱法（GC-HS）分析艾草烟成分。结论艾草烟对中枢神经系统有浓度依赖性的舒张作用。此外，GC-HS分析表明，艾草烟中浓度最高的成分是杜仲醇，这是一种因其对中枢神经系统的舒缓作用而被广泛认可的精油。因此，这可能是艾草烟的镇静作用的原因。
OBJECTIVETo evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye (Folium Artemisiae Argyi) has on the central nervous system.METHODSForty Kunming mice (half male) were randomly assigned (n= 10/group) to a control group (C) or one of three moxa smoke concentration groups (% opacity): low (L1; 0.4%), medium (M1; 2%), and high (H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for 7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium (PS)-injected group, M1 +PS group, H1 (15%) + PS group, and a positive control group (10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration, and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice, and the composition of moxa smoke was analyzed using headspace gas chromatography (GC-HS).RESULTSA low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSIONThe concentration-dependent relaxing effects of moxa smoke on the Central Nervous System (CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyptol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.