Incomplete transient ischemia: a non-destructive evaluation of in vivo cerebral metabolism and hemodynamics in rat brain.
Incomplete transient ischemia: a non-destructive evaluation of in vivo cerebral metabolism and hemodynamics in rat brain.
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不完全短暂性缺血：大鼠脑体内脑代谢和血流动力学的无损评估。
DOI：
10.1161/01.str.12.6.864
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发表时间：
1981
期刊：
影响因子：
8.3
通讯作者：
Jöbsis,FF
中科院分区：
文献类型：
--
作者：
Wiernsperger,N;Sylvia,AL;Jöbsis,FF
差示近红外分光光度法用于监测不完全短暂性缺血（急性可逆性颈总动脉闭塞）期间和之后脑血红蛋白饱和度、血容量和细胞色素c氧化酶还原/氧化反应的连续体内变化。在这项研究中，通过透照法对完整头骨中的大鼠大脑进行非侵入性监测。数据表明，颈动脉结扎后脑血红蛋白脱氧作用的增加与血容量的减少同时发生，作为一种补偿机制，有助于维持有氧能量代谢。观察结果还表明，在该物种中，双侧颈动脉闭塞对脑血管参数的影响不一定是不可逆转的。只要全身动脉血压不低于约 40 mm Hg 的值，通过细胞色素 c 氧化酶氧化还原转变评估的线粒体内代谢改变是可逆的。这些数据表明，在高能量储存发生重大变化之前，可以使用细胞色素c氧化酶的临界降低水平作为缺血引起的脑代谢功能障碍的早期指示。
Differential near infrared spectrophotometry was used to monitor sequential in vivo alterations in cerebral hemoglobin saturation, blood volume, and cytochrome c oxidase reduction/oxidation responses during and after a period of incomplete transient ischemia (acute, reversible common carotid artery occlusion). In this study the rat brain was monitored non-invasively in the intact skull by transillumination. The data show that an increase in cerebral deoxygenation of hemoglobin, which occurs simultaneously with a decrease in blood volume subsequent to carotid ligation, acts as a compensatory mechanism to assist in maintaining aerobic energy metabolism. The observations also demonstrate that in this species the effects of bilateral carotid occlusion on the cerebrovascular parameters are not necessarily irrevocable. The intramitochondrial metabolic alterations, as evaluated by cytochrome c oxidase redox transitions, are reversible as long as the systemic arterial blood pressure does not fall below a value of approximately 40 mm Hg. These data suggest that possibility of being able to use a critical reduction level of cytochrome c oxidase as an early indication of ischemia-induced cerebral metabolic dysfunction prior to major changes in high energy stores.