Plasticity of tyrosine hydroxylase and serotonergic systems in the regenerating spinal cord of adult zebrafish
Plasticity of tyrosine hydroxylase and serotonergic systems in the regenerating spinal cord of adult zebrafish
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DOI：
10.1002/cne.22739
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发表时间：
2012-04-01
影响因子：
2.5
通讯作者：
Becker, Thomas
中科院分区：
文献类型：
--
作者：
Kuscha, Veronika;Barreiro-Iglesias, Anton;Becker, Thomas
脊髓的单胺能神经支配对运动具有重要的调节功能。在这里，我们进行了一项定量研究，以确定成年斑马鱼脊髓成功再生过程中酪氨酸羟化酶阳性（TH1+；主要是多巴胺能）和血清素能（5-HT+）末端和细胞的可塑性变化。脊髓中的 TH1+ 神经支配源自大脑。脊髓横断后，尾部脊髓的 TH1+ 免疫反应性完全丧失。与未病变对照相比，病变部位头端末端静脉曲张的密度增加，并在病变后 6 周在尾部脊髓中重新建立。有趣的是，即使在延长存活时间后，轴突也大多无法重新支配脊髓的尾部水平。然而，末端静脉曲张的密度与游泳行为的恢复相关，而游泳行为在脊髓再次损伤后又完全丧失。对于来自大脑的下降 5-HT+ 轴突的末端也进行了类似的观察。此外，脊髓 5-HT+ 神经元在病变后新产生，数量短暂增加至五倍，这在一定程度上取决于刺猬信号传导。总体而言，成年斑马鱼成功再生的脊髓中 TH1+ 和 5-HT+ 神经支配发生了巨大改变。尽管 TH 和 5-HT 系统发生这些变化，但游泳能力仍显着恢复，这表明成人脊柱网络在再生过程中具有显着的可塑性。 J.Comp。内罗尔。 520:933951, 2012。(C) 2011 Wiley 期刊公司。
Monoaminergic innervation of the spinal cord has important modulatory functions for locomotion. Here we performed a quantitative study to determine the plastic changes of tyrosine hydroxylase-positive (TH1+; mainly dopaminergic), and serotonergic (5-HT+) terminals and cells during successful spinal cord regeneration in adult zebrafish. TH1+ innervation in the spinal cord is derived from the brain. After spinal cord transection, TH1+ immunoreactivity is completely lost from the caudal spinal cord. Terminal varicosities increase in density rostral to the lesion site compared with unlesioned controls and are re-established in the caudal spinal cord at 6 weeks post lesion. Interestingly, axons mostly fail to re-innervate more caudal levels of the spinal cord even after prolonged survival times. However, densities of terminal varicosities correlate with recovery of swimming behavior, which is completely lost again after re-lesion of the spinal cord. Similar observations were made for terminals derived from descending 5-HT+ axons from the brain. In addition, spinal 5-HT+ neurons were newly generated after a lesion and transiently increased in number up to fivefold, which depended in part on hedgehog signaling. Overall, TH1+ and 5-HT+ innervation is massively altered in the successfully regenerated spinal cord of adult zebrafish. Despite these changes in TH and 5-HT systems, a remarkable recovery of swimming capability is achieved, suggesting significant plasticity of the adult spinal network during regeneration. J. Comp. Neurol. 520:933951, 2012. (C) 2011 Wiley Periodicals, Inc.