Ledgerline, a novel Xenopus laevis gene, regulates differentiation of presomitic mesoderm during somitogenesis
Ledgerline, a novel Xenopus laevis gene, regulates differentiation of presomitic mesoderm during somitogenesis
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DOI：
10.2108/zsj.23.689
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发表时间：
2006-08-01
影响因子：
0.9
通讯作者：
Asashima, Makoto
中科院分区：
文献类型：
--
作者：
Chan, Techuan;Satow, Reiko;Asashima, Makoto
通过体节的顺序形成来分割脊椎动物的身体是胚胎发生中的一个重要过程。这一过程由成纤维细胞生长因子和维甲酸(RA)信号对Notch相关分子振荡器的激活和调节所支配。在这项研究中，我们发现了非洲爪哇的一个新基因ledgerfinin，该基因在非洲爪哇的中胚层中特异表达。使用反义吗啉寡核苷酸敲除来吉芬使发育中的体节前锋发生改变，并改变了调节分子振荡的基因的表达，包括Delta2、ESR5、Hairy2a和Thylacinel。敲除雷吉林也可下调RALDH-2的表达。注射RARα-CA，RARα-CA是RA受体RARA的结构性活性突变体，随后减少改变的胸腺细胞表达。这些结果有力地表明，来吉芬在非洲爪哇体细胞发生过程中对中胚层RA活性和中胚层发育前期中胚层的分化至关重要。
Segmentation of the vertebrate body via the sequential formation of somites is an important process in embryogenesis. This sequential process is governed by the activation and regulation of Notch-related molecular oscillators by fibroblast growth factor and retinoic acid (RA) signaling. In this study, we identified ledgerfine, a novel gene of Xenopus laevis expressed specifically in the presomitic mesoderm. Knockdown of ledgerfine using antisense morpholino oligonucleotides shifted the developing somite front and altered the expression of genes that regulate molecular oscillation, including Delta2, ESR5, Hairy2a, and Thylacinel. Knockdown of ledgerline also downregulated RALDH-2 expression. Injection of RAR alpha-CA, a constitutively active mutant of the RA receptor RARa, subsequently reduced the altered Thylacinel expression. These results strongly suggest that ledgerfine is essential for mesodermal RA activity and differentiation of the presomitic mesoderm during Xenopus somitogenesis.