Mcl-1 and Bcl-xL are co-regulated by IL-6 in human myeloma cells
Mcl-1 and Bcl-xL are co-regulated by IL-6 in human myeloma cells
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DOI：
10.1046/j.1365-2141.1999.01705.x
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发表时间：
1999-11-01
影响因子：
6.5
通讯作者：
Amiot, M
中科院分区：
文献类型：
--
作者：
Puthier, D;Derenne, S;Amiot, M
多发性骨髓瘤（MM）是一种缓慢增生的恶性肿瘤，恶性浆细胞在骨髓内积聚。免疫印迹法测定了几种抗凋亡蛋白在人骨髓瘤细胞系和高度纯化的天然骨髓瘤细胞中的表达。Bcl-x(L)、Mcl-1和Bcl-2在大部分样品中均有表达；Bcl-x(L)和Mcl-1的表达似乎与骨髓瘤细胞有关。在骨髓瘤细胞因生长因子剥夺而凋亡的系统中，我们发现Bcl-2的作用似乎很小，而Mcl-1和Bcl-x(L)则受到白细胞介素(IL)-6的严格调节。这些发现强调了Mcl-1和Bcl-x(L)而不是Bcl-2在il -6诱导的骨髓瘤细胞存活中的重要作用。
Multiple myeloma (MM) is a slowly proliferative malignancy in which malignant plasma cells accumulate within the bone marrow. The expression of several anti-apoptotic proteins was evaluated by immunoblotting in human myeloma cell lines and in highly purified native myeloma cells. Expression of Bcl-x(L), Mcl-1 and Bcl-2 was found in most of the samples; expression of Bcl-x(L) and Mcl-1 seemed to be related on myeloma cells. In a system of apoptosis by growth factor deprivation on myeloma cells, we showed that the effect of Bcl-2 seemed minimal whereas Mcl-1 and Bcl-x(L) were tightly regulated by interleukin (IL)-6. These findings underline the important role of Mcl-1 and Bcl-x(L) instead of Bcl-2 in IL-6-induced survival of myeloma cells.