The role of modifications in codon discrimination by tRNALys UUU
The role of modifications in codon discrimination by tRNALys UUU
复制标题
DOI：
10.1038/nsmb861
复制
发表时间：
2004-12-01
影响因子：
16.8
通讯作者：
Agris, PF
中科院分区：
文献类型：
--
作者：
Murphy, FV;Ramakrishnan, V;Agris, PF
许多tRNA中特定核苷的天然修饰在核糖体对mRNA的解码过程中是必不可少的。例如，tRNA（UUU）（Lys）需要在第37位（t（6）A37）的修饰N6-苏氨酰氨甲酰基腺苷，与反密码子的第3位相邻，以结合核糖体30 S亚基A位点的AAA。此外，当在摆动位置（mnm（5）U34或s（2）U34）用t（6）A37和5-甲氨基甲基尿苷或2-硫代尿苷双重修饰时，它只能结合AAA和AAG赖氨酸密码子。在这里，我们报告晶体结构的修改tRNA反密码子茎环绑定到30 S核糖体亚基与赖氨酸密码子在A位点。这些结构允许反密码子环中的修饰如何使赖氨酸密码子AAA和AAG解码合理化。
The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(UUU)(Lys) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3 to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.