Expression of glycolytic enzymes is increased in pancreatic cancerous tissues as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry.
Expression of glycolytic enzymes is increased in pancreatic cancerous tissues as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry.
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DOI：
10.3892/ijo.30.4.849
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发表时间：
2007-04
影响因子：
5.2
通讯作者：
Kuniko Mikuriya;Y. Kuramitsu;S. Ryozawa;M. Fujimoto;Sayaka Mori;M. Oka;K. Hamano;K. Okita;I. Sakaida;K. Nakamura
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文献类型：
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作者：
Kuniko Mikuriya;Y. Kuramitsu;S. Ryozawa;M. Fujimoto;Sayaka Mori;M. Oka;K. Hamano;K. Okita;I. Sakaida;K. Nakamura
胰腺癌患者由于诊断较晚，对各种治疗方法缺乏反应，预后很差。我们试图通过胰腺癌组织的蛋白质组学图谱来确定可能用于早期诊断和有效治疗的蛋白质。用双向凝胶电泳法对10例胰腺癌患者手术切除或尸检获得的胰腺癌组织和配对的非肿瘤组织进行分析。差异显示有11个点在癌组织中的表达比配对的非癌组织中的表达增强。经LC-MS/MS系统鉴定为：α-烯醇化酶、甘油醛-3-磷酸脱氢酶、磷酸丙糖异构酶、转明胶、钙调蛋白、超氧化物歧化酶线粒体前体、谷胱甘肽S转移酶P、亲环素A、蛋白二硫键异构酶A3前体和载脂蛋白A-I前体。在11个斑点中，有两个被检测为GAPDH。我们注意到11个点中有4个是参与糖酵解途径的酶。肿瘤细胞糖酵解增加被认为是肿瘤内缺氧的结果，可能与肿瘤的侵袭、转移或对治疗的抵抗有关。Western blotting和免疫组织化学证实了这些糖酵解蛋白和转明胶的存在。
The prognosis of patients with pancreatic cancer is very poor because of late diagnosis and the lack of response to various therapies. We tried to identify proteins that might be available for early diagnosis and effective therapies by proteomic profiling of pancreatic cancer tissues. Pancreatic cancerous and paired non-cancerous tissues obtained from surgical resections or autopsies of 10 patients were analyzed by two-dimensional gel electrophoresis. The differential display showed 11 spots whose expression was increased in cancerous tissues compared with the paired non-cancerous tissues. The liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) system identified the spots as alpha-enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), triosephosphate isomerase, transgelin, calmodulin, superoxide dismutase(Mn) mitochondrial precursor, glutathione S-transferase P, cyclophilin A, protein disulfide isomerase A3 precursor, and apolipoprotein A-I precursor. Two of the 11 spots were detected as GAPDH. We noticed that 4 of 11 spots were enzymes involved in glycolytic pathway. Increased glycolysis in cancer cells has been regarded as the effect of intratumoral hypoxia and is possibly associated with tumor invasion, metastasis or resistance to therapies. These glycolytic proteins and transgelin, were confirmed by Western blotting and immunohistochemistry.