Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue
Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue
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HMG 对小鼠卵巢组织异位自体移植血运重建和卵泡存活的影响
DOI：
10.1016/j.rbmo.2012.02.025
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Jiang, Xiaohua
中科院分区：
文献类型：
--
作者：
Wang, Yanrong;Chang, Qing;Jiang, Xiaohua
卵巢组织移植现在被认为是一个程序，以保持生育能力的年轻女性患者接受癌症治疗。本研究探讨绝经期促性腺激素（HMG）对自体异位卵巢移植血管重构的影响及其机制。用不同浓度的HMG体外培养8周龄小鼠卵巢3 h，检测卵巢组织中血管内皮生长因子（VEGF）的表达。将培养的卵巢植入肾被膜下，于移植后24、36、48 h或1个月取出。观察卵巢血运重建情况、液体渗出情况和存活卵泡数。结果显示，HMG干预组VEGF增加了1.6-6.5倍。移植后24-36 h出现血运重建，早于对照组。随着HMG浓度的增加，液体渗出逐渐增加。移植后1个月，HMG 0.15IU/ml组的存活卵泡数较其他各组增加1.2-1.5倍。结论：移植前给予HMG体外干预可改善自体移植卵泡的血供重建和存活，其机制可能与VEGF表达增加有关。(C)2012年，爱思唯尔有限公司出版的生殖保健有限公司。版权所有。
Ovarian tissue transplantation is now considered as a procedure to preserve the fertility of young women patients undergoing cancer therapy. The present study investigated the effects and mechanism of human menopausal gonadotrophin (HMG) intervention on vascular remoulding in ovarian heterotopic autotransplantation. Ovaries of 8-week-old mice were cultured in vitro with different concentrations of HMG for 3 h for measuring the expression of vascular endothelial growth factor (VEGF). The cultured ovaries were implanted under the kidney capsule and removed 24, 36, 48 h or 1 month after transplantation. Revascularization, fluid exudation and the number of surviving ovarian follicles were observed. The results showed that VEGF was increased 1.6-6.5 times in the HMG intervention groups. Revascularization appeared 24-36 h after transplantation and was earlier than that of the control. Fluid exudation increased incrementally with increasing HMG concentrations. The total number of surviving ovarian follicles was increased by 1.2-1.5 times in the HMG 0.15 IU/ml group as compared with the other groups 1 month after transplantation. It is concluded that intervention with HMG in vitro before transplantation could improve the blood supply reconstruction and survival of the autotransplanted ovarian follicles, which might be associated with increased VEGF expression. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.