Influence of Tobacco Variety and Curing on Free Radical Production in Cigarette Smoke.
Influence of Tobacco Variety and Curing on Free Radical Production in Cigarette Smoke.
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烟草品种和烘烤对卷烟烟雾中自由基产生的影响。
DOI：
10.1093/ntr/ntad049
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Richie,JohnP
中科院分区：
文献类型：
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作者：
Bitzer,ZacharyT;Mocniak,LeanneE;Trushin,Neil;Smith,Michael;Richie,JohnP
香烟烟雾中含有高度活性的自由基，被认为在烟草烟雾引起的危害中起重要作用。以前，在商业香烟中观察到自由基和毒物输出的巨大变化。这些变化可能是由于卷烟的设计特点（纸张、过滤嘴和添加剂）、烟草品种（白烟、亮烟、东方烟等）和烟草烘烤方法（空气、太阳、烟道和火）。以前的报告表明，烟草品种和烘烤方法会影响烟草烟雾成分的产生，如烟草特有的致癌物质尼古丁衍生亚硝胺酮（NNK）。目的和方法我们评估了用15种不同类型烟草生产的卷烟烟雾中自由基、尼古丁和NNK的产生。采用n -叔丁基-α-苯基硝基酮自旋俘获法捕获气相自由基，在剑桥滤垫（CFP）上捕获颗粒相自由基。用电子顺磁共振谱分析了这两种自由基。采用气相色谱-火焰电离检测法和液相色谱-质谱联用法分别对烟碱和NNK进行分析。结果不同烟草类型的气相自由基含量相差近8倍，其中圣詹姆斯·佩里克烟草气相自由基含量最高（42±7 nmol/g），加拿大弗吉尼亚烟草气相自由基含量最低（5±2 nmol/g）。烟雾中的尼古丁和NNK含量按类型分别变化14倍和192倍。气相自由基与NNK水平高度相关（r= 0.92,p< 0.0001），且受烟草烘烤方式的影响最大。综上所述，这些数据表明，卷烟生产中使用的烟草类型可以作为减少吸烟危害的监管目标。卷烟自由基和NNK水平的变化因烟草品种和烘烤方法而异。减少高产自由基和NNK烟草类型的比例，为监管机构和生产商寻求减少卷烟中有毒物质的排放提供了一个潜在的工具。
IntroductionCigarette smoke contains highly reactive free radicals thought to play an important role in tobacco smoke-induced harm. Previously, large variations in free radical and toxicant output have been observed in commercial cigarettes. These variations are likely because of cigarette design features (paper, filter, and additives), tobacco variety (burley, bright, oriental, etc.), and tobacco curing methods (air, sun, flue, and fire). Previous reports show that tobacco varieties and curing methods influence the production of tobacco smoke constituents like the tobacco-specific carcinogen nicotine-derived nitrosamine ketone (NNK).Aims and MethodsWe evaluated free radical, nicotine, and NNK production in cigarette smoke from cigarettes produced with 15 different types of tobacco. Gas-phase free radicals were captured by spin trapping with N-tert-butyl-α-phenylnitrone and particulate-phase radicals were captured on a Cambridge Filter pad (CFP). Both types of radicals were analyzed using electron paramagnetic resonance spectroscopy. Nicotine and NNK were extracted from the CFP and analyzed by gas chromatography flame ionization detection and liquid chromatography-mass spectrometry, respectively.ResultsGas-phase radicals varied nearly 8-fold among tobacco types with Saint James Perique tobacco producing the highest levels (42 ± 7 nmol/g) and Canadian Virginia tobacco-producing the lowest levels (5 ± 2 nmol/g). Nicotine and NNK levels in smoke varied 14-fold and 192-fold, respectively, by type. Gas-phase free radicals were highly correlated with NNK levels (r= 0.92,p< .0001) and appeared to be most impacted by tobacco curing method.ConclusionsAltogether, these data suggest that tobacco types used in cigarette production may serve as a target for regulation to reduce harm from cigarette smoking.ImplicationsVariations in cigarette free radical and NNK levels vary based on the tobacco variety and curing method. Reducing the ratio of high-producing free radical and NNK tobacco types offer a potential tool for regulators and producers looking to reduce toxicant output from cigarettes.